OLDEST-OLD MORTALITY AND DISABILITY FOR DROSOPHILA

果蝇的高龄死亡率和残疾

• 批准号: 6234229
• 负责人: JAMES W CURTSINGER
• 金额: $ 25.97万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 1997-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6234229
• 关键词: Drosophilidae; animal genetic material tag; animal mortality; animal old age; animal population genetics; gender difference; genetic mapping; genetic markers; longevity; mathematical model; quantitative trait loci

We propose to investigate mortality and morbidity at the oldest ages in Drosophila melanogaster, an experimental system that is well-suited to laboratory analysis and genetic manipulation. Specific aims are: 1) QTL Mapping- We will estimate the number, chromosomal location, and magnitude of effects of genes that cause variation in longevity using DNA-based genetic markers. 2) Demography of Genes- We will estimate age-dependent mortality parameters for small chromosomal segments carried in genetically homogeneous recombinant inbred lines. 3) ADL Experiments- We will characterize age-dependent disability in cohorts of genetically homogeneous flies, and estimate the genetic correlation of mortality and morbidity across genotypes. Notable features are: (i) The experiments emphasize large scale data collection for estimation of genetic and demographic parameters. (ii) QTL mapping experiments employ some of the most recent developments in DNA technology (PCR and RAPDs). (iii) Data will be informative about the genetic basic of male-female correlations. (iv) Data will be archive for general access. Health relevance: The research will provide information about the plasticity of morbidity and mortality at the oldest ages in a model experimental system.

我们建议调查死亡率和发病率在最古老的年龄， 果蝇，一个非常适合于 实验室分析和基因操作。 具体目标是： 1)QTL作图-我们将估计数量，染色体位置， 基因对寿命的影响程度 基于DNA的遗传标记。 2)基因的人口统计学-我们将估计年龄相关的死亡率 遗传学上携带的小染色体片段的参数 同源重组近交系 3)ADL实验-我们将描述年龄依赖性残疾， 基因同质的苍蝇，并估计遗传 不同基因型之间的死亡率和发病率的相关性。 显著特点是：（i）实验强调大规模数据 收集用于估计遗传和人口统计学参数的数据。(ii)QTL 绘图实验采用了DNA领域的一些最新进展， 技术（PCR和RAPD）。(iii)数据将提供有关 雌雄相关的遗传基础。(iv)数据将存档， 一般访问。 健康相关性：研究将提供有关 模型中最老年龄发病率和死亡率的可塑性 实验系统