OLDEST-OLD MORTALITY FOR INBRED LINES OF DROSOPHILA

果蝇近交系最年老死亡率

• 批准号: 3790344
• 负责人: JAMES W CURTSINGER
• 金额: --
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3790344
• 关键词: Drosophilidae; animal mortality; animal old age; animal population genetics; computer data analysis; genetic models; genetic strain; homozygote; inbreeding; longevity; mathematical model

The objective of the proposed experiments is to provide data in a model experimental system for evaluation of the theory of rectangularization of survivorship. This theory and the general alternative view make drastically different predictions about the age-structure of human populations in the next century. Specific objectives are: (i) To measure survivorship among the oldest survivors of 50,000 flies from inbred lines of Drosophila melanogaster. These data will allow accurate determination of the force of mortality at extreme ages, and a test for the existence of genotype-specific "caps" on longevity. (ii) To obtain survivorship data on the oldest of 50,000 flies derived from crosses between inbred lines. These data will provide further information about the existence of caps, independent of biases that arise as a result of complete homozygosity in inbred lines. (iii) To test a heterogeneous population of Drosophila for the presence of a critical prerequisite for the evolution of a rectangularized survivorship curve: genetic variation that promotes age-specific mortality. This will be investigated by an artificial selection experiment. (iv) To construct an archive of the data collected in the above experiments for the use of other biologists interested in survivorship and genetic variability of aging. (v) To analyze the data on the 50,000 files in experiments 1 and 2 using methods of life-table analysis and survival analysis to answer some specific questions of cross-cutting relevance to this program project.

拟议实验的目的是提供数据， 理论评价模型实验系统 生存矩形化 这个理论和一般的 另一种观点对未来的预测大相径庭。 下个世纪人口的年龄结构。 具体目标是： （一） 为了衡量5万名最年长的幸存者的生存率， 来自黑腹果蝇近交系的苍蝇。 这些数据 将允许准确确定死亡力， 极端的年龄，并测试基因型特异性的存在， 长寿的“上限”。 (ii)为了获得50，000只苍蝇中最老的苍蝇的生存数据， 由近交系之间的杂交衍生而来。 这些数据将提供 关于是否存在上限的进一步资料， 由于近交系中完全纯合性而产生的偏差 线 (iii)为了测试果蝇的异质种群， 一个进化的关键先决条件的存在 矩形化生存曲线：遗传变异促进 年龄别死亡率 这将由一个人工的 选择实验 (iv)将上述收集的数据建立档案， 其他生物学家感兴趣的实验， 存活率和衰老的遗传变异性。 （五） 为了分析实验1和实验2中50，000个文件的数据， 2运用寿命表分析和生存分析方法， 回答与此有关的一些跨领域具体问题 程序项目。