OLDEST-OLD MORTALITY AND DISABILITY FOR DROSOPHILA

我们建议调查最大年龄组的死亡率和发病率 果蝇，一个非常适合的实验系统 实验室分析和基因操作。 具体目标是： 1) QTL作图-我们将估计数量、染色体位置和 导致寿命变化的基因影响的大小 基于 DNA 的遗传标记。 2) 基因人口统计学-我们将估计年龄相关的死亡率 遗传携带的小染色体片段的参数 同质重组自交系。 3) ADL 实验——我们将描述年龄依赖性残疾的特征 遗传同质的果蝇队列，并估计遗传 不同基因型死亡率和发病率的相关性。 显着特点是：（i）实验强调大规模数据 用于估计遗传和人口参数的收集。 (二)QTL 绘图实验采用了 DNA 的一些最新进展 技术（PCR 和 RAPD）。 (iii) 数据将提供有关 男女相关性的遗传基础。 (iv) 数据将被存档 一般访问。 健康相关性：该研究将提供有关以下方面的信息： 模型中最高年龄发病率和死亡率的可塑性 实验系统。

We propose to investigate mortality and morbidity at the oldest ages in Drosophila melanogaster, an experimental system that is well-suited to laboratory analysis and genetic manipulation. Specific aims are: 1) QTL Mapping- We will estimate the number, chromosomal location, and magnitude of effects of genes that cause variation in longevity using DNA-based genetic markers. 2) Demography of Genes- We will estimate age-dependent mortality parameters for small chromosomal segments carried in genetically homogeneous recombinant inbred lines. 3) ADL Experiments- We will characterize age-dependent disability in cohorts of genetically homogeneous flies, and estimate the genetic correlation of mortality and morbidity across genotypes. Notable features are: (i) The experiments emphasize large scale data collection for estimation of genetic and demographic parameters. (ii) QTL mapping experiments employ some of the most recent developments in DNA technology (PCR and RAPDs). (iii) Data will be informative about the genetic basic of male-female correlations. (iv) Data will be archive for general access. Health relevance: The research will provide information about the plasticity of morbidity and mortality at the oldest ages in a model experimental system.