CLINICAL EXPERIMENT OF HELICOBACTER PYLORI TRANSMISSION

描述（取自应用程序） 幽门螺杆菌感染是最常见的细菌感染之一 在世界范围内造成严重的发病率和死亡率。然而，为了 迄今为止，我们还不知道这种生物是如何传播的。虽然大多数数据 来自美国、欧洲和澳大利亚的支持直接个人对个人的 传播，这种情况发生的方式是未知的。我们假设 通过直接接触呕吐物或雾化经胃-口传播 呕吐物是最有可能的传播途径我们进一步 假设胃低氯血症可能促进活性 有机体本研究的具体目的是评估是否可行 H.幽门螺杆菌存在于呕吐物中， 呕吐、唾液和/或粪便。为了做到这一点，我们打算进行一项临床研究， 实验时间为30 H。pylori感染者经H. 使用血清学和13 C尿素呼气试验检测幽门螺杆菌感染，受试者将 提供H的唾液和粪便样本幽门螺杆菌检测其中一半 然后受试者将用西咪替丁治疗两周， 不接受组胺拮抗剂。在此期间结束时，所有受试者 将被斯坦福大学的临床研究中心录取， 然后依次诱发呕吐粪便、呕吐物、唾液和空气 将对样本进行H检测。pylori四种方法：常规培养， 免疫磁性分离后培养，免疫磁性分离 随后进行PCR和常规PCR（仅空气过滤器样品）。H. 将比较来自呕吐物、空气、粪便和唾液的幽门螺杆菌。 目前，卫生干预措施有关H。幽门螺杆菌感染 治疗H.幽门相关疾病，而不是预防感染。 预防性疫苗的可用性仍然需要数年至数十年的时间。 了解H的路径。幽门螺旋杆菌的传播是一个关键的起点 疾病预防的重点，并将使更多的针对性， 干预策略。

DESCRIPTION (taken from the application) Helicobacter pylori infection is among the most common bacterial infections of man, causing significant morbidity and mortality worldwide. Yet, to date, we do not understand how the organism is transmitted. While most data from the United States, Europe and Australia support direct person-to-person spread, the manner in which this occurs is unknown. We hypothesize that gastric-oral transmission via direct contact with vomitus or aerosolization of vomitus is the most probable means of transmission. We further hypothesize that gastric hypochlorhydria may foster excretion of viable organisms. The specific aims of this study are to evaluate whether viable H. pylori organisms are present in vomitus, air samples obtained during vomiting, saliva and/or feces. To do this, we intend to conduct a clinical experiment in 30 H. pylori infected volunteers. After confirmation of H. pylori infection using serology and 13C urea breath test, subjects will provide saliva and a stool sample for H. pylori testing. One half of these subjects will then be treated with cimetidine for two weeks while the others receive no histamine antagonist. At the end of this period, all subjects will be admitted to the Clinical Research Unit at Stanford where catharsis and emesis will be sequentially induced. Stool, vomit, saliva and air samples will be tested for H. pylori four ways: routine culture, immunomagnetic separation followed by culture, immunomagnetic separation followed by PCR and routine PCR (air filter samples only). The yield of H. pylori from vomitus, air, feces and saliva will be compared. Currently, health interventions related to H. pylori infection involve treatment of H. pylori-related diseases rather than prevention of infection. Availability of prophylactic vaccines remains years to decades away. Understanding the route(s) of H. pylori transmission is a critical starting point for disease prevention and will enable more directed targeting of intervention strategies.