MECHANISM OF TERMINATION OF DNA REPLICATION

描述（改编自申请人摘要）： 复制叉、复制解旋酶和RNA链的极性停滞 由细菌复制终止蛋白引起的延伸将是 利用X射线晶体学、诱变和生物化学 终止子蛋白的突变形式的分析。 实验 应该能提供明确的证据证明DNA蛋白质 末端DNA和终止子蛋白之间的相互作用， 蛋白质-蛋白质接触之间的逮捕和逮捕的蛋白质 导致复制终止。 A的生理作用 终止子蛋白作为复制的检查点，将通过 分子克隆和体内分析。 一个基因选择方案将是 用于尝试克隆和研究来自真核细胞的基因， 复制叉阻止蛋白 X射线晶体学将用于 确定末端-终止子蛋白复合物的结构。

DESCRIPTION (Adapted from applicant's abstract): The molecular mechanism of polar arrest of replication forks, replicative helicases and RNA chain elongation by the bacterial replication terminator proteins will be investigated using x-ray crystallography, mutagenesis and biochemical analysis of the mutant forms of the terminator proteins. The experiments should provide definitive evidence as to whether not only DNA protein interaction between the terminus DNA and the terminator protein but also protein-protein contacts between the arresting and the arrested protein contributed to replication termination. The physiological role of a terminator protein as checkpoints of replication will be investigated by molecular cloning and in vivo analysis. A genetic selection scheme will be used to attempt to clone and study genes from eukaryotic cells that encode replication fork-arresting protein. X-ray crystallography will be use to determine the structure of a terminus-terminator protein complex.