MECHANISM OF TERMINATION OF DNA REPLICATION

描述(改编自申请者的摘要)： 复制分叉、复制解旋酶和核糖核酸链的极性停滞 细菌复制终止子蛋白的延长将是 用x射线结晶学、诱变和生化研究 终止子蛋白的突变形式分析。这些实验 应该提供确凿的证据，证明不仅仅是DNA蛋白质 末端DNA与终止子蛋白的相互作用 捕获物和被捕获物之间的蛋白质-蛋白质接触 有助于终止复制。一个人的生理作用 终止子蛋白作为复制的检查点将由 分子克隆和体内分析。基因选择方案将是 用于尝试从真核细胞中克隆和研究编码 复制分叉抑制蛋白。X射线结晶学将用于 确定末端-终止子蛋白复合体的结构。

DESCRIPTION (Adapted from applicant's abstract): The molecular mechanism of polar arrest of replication forks, replicative helicases and RNA chain elongation by the bacterial replication terminator proteins will be investigated using x-ray crystallography, mutagenesis and biochemical analysis of the mutant forms of the terminator proteins. The experiments should provide definitive evidence as to whether not only DNA protein interaction between the terminus DNA and the terminator protein but also protein-protein contacts between the arresting and the arrested protein contributed to replication termination. The physiological role of a terminator protein as checkpoints of replication will be investigated by molecular cloning and in vivo analysis. A genetic selection scheme will be used to attempt to clone and study genes from eukaryotic cells that encode replication fork-arresting protein. X-ray crystallography will be use to determine the structure of a terminus-terminator protein complex.