QUANTITATIVE STUDIES OF THE BIOTIN REGULATORY SYSTEM
生物素调节系统的定量研究
基本信息
- 批准号:2701549
- 负责人:
- 金额:$ 10.9万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1992
- 资助国家:美国
- 起止时间:1992-05-01 至 1999-01-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Regulation of the expression of genetic information is critical to the
proper development and functioning of any organsim. Regulation at
the level of transcription initiation has been demonstrated to depend on
macromolecular interactions formed between multiple proteins and
target sites on the DNA. A mechanistic understanding of
transcriptional regulation therefore requires detailed quantiative
information about the individual macromolecular interactions that
contribute to the process. The biotin regulatory system from
Escherichia coli is an example of a complex transcriptional regulatory
system in which physiological function depends on a combination of
protein-small molecule, homologous and heterologous protein-protein
and protein-DNA interactions. Thermodynamic coupling moreover,
exists between sthe individual interactions. The goals of this reserch
are to elucidate the structural, thedrmodynamic and kinetic rules that
govern the physiological function in the biotin transcriptional regulatory
system. The kinetics of cooperative binding of the major regulatory
protein, the bioyin repressor, to its target site on the DNA, the biotin
operator, will be measured using Time Resolved Dnase Footprinting
and Stopped-Flow Fluorescence. Direct Equilbrium Fluorescence
Titrations and Equilibrium Analytical Ultracentrigugation
Measurements will be utilized to examine coupling of DNA binding to
protein dimerization in assembly of the transcriptional repression
complex. The structural elements of the protein and DNA involved in
the multiple macromolecular interfaces formed in the transcriptional
repression complex will be probed using Chemical DNA Footprinting
and Chemical Protein Footprinting techniques. Single site mutants that
disrupt one or more of these interfaces will be isolated and
characterized o quantitate the consequences of altrations in the
structural integrity of each interface for the functional energetics of the
system. Finally, crystallization of complexes of the biotin repressor
bound to small ligands and to DAN is proposed so that we may
ultimately obtain high resolution structures of the multiple functional
states of this allosteric site-specific DNA binding protein. Results of
these proposed studies of the biotin regulatory system will provide
information that is relevant to understanding the mechanism of function
of any complex transcriptional regulatory system.
基因信息表达的调控对细胞发育至关重要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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DOROTHY BECKETT其他文献
DOROTHY BECKETT的其他文献
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{{ truncateString('DOROTHY BECKETT', 18)}}的其他基金
Signal Propagation in Protein Allostery: Mechanism and Evolution
蛋白质变构中的信号传播:机制和进化
- 批准号:
9900030 - 财政年份:2019
- 资助金额:
$ 10.9万 - 项目类别:
Purchase of a Beckman XL-1 Analytical Ultracentrifuge
购买 Beckman XL-1 分析超速离心机
- 批准号:
6440908 - 财政年份:2002
- 资助金额:
$ 10.9万 - 项目类别:
BIOTIN CARBOXY TERMINAL DOMAIN FOLDING CALORIMETRY E COLI ACETYL COA CARBOXYLASE
生物素羧基末端折叠量热法 大肠杆菌乙酰辅酶A羧化酶
- 批准号:
6122028 - 财政年份:1997
- 资助金额:
$ 10.9万 - 项目类别:
QUANTITATIVE STUDIES OF THE BIOTIN REGULATORY SYSTEM
生物素调节系统的定量研究
- 批准号:
2184011 - 财政年份:1992
- 资助金额:
$ 10.9万 - 项目类别:
QUANTITATIVE STUDIES OF THE BIOTIN REGULATORY SYSTEM
生物素调节系统的定量研究
- 批准号:
2184010 - 财政年份:1992
- 资助金额:
$ 10.9万 - 项目类别:
QUANTITATIVE STUDIES OF THE BIOTIN REGULATORY SYSTEM
生物素调节系统的定量研究
- 批准号:
2022498 - 财政年份:1992
- 资助金额:
$ 10.9万 - 项目类别:
QUANTITATIVE STUDIES OF THE BIOTIN REGULATORY SYSTEM
生物素调节系统的定量研究
- 批准号:
6028316 - 财政年份:1992
- 资助金额:
$ 10.9万 - 项目类别:
Quantitative Studies of the Biotin Regulatory System
生物素调节系统的定量研究
- 批准号:
7654285 - 财政年份:1992
- 资助金额:
$ 10.9万 - 项目类别:
Quantitative Studies of the Biotin Regulatory System
生物素调节系统的定量研究
- 批准号:
7228636 - 财政年份:1992
- 资助金额:
$ 10.9万 - 项目类别:
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