Purchase of a Beckman XL-1 Analytical Ultracentrifuge

购买 Beckman XL-1 分析超速离心机

• 批准号: 6440908
• 负责人: DOROTHY BECKETT
• 金额: $ 21.5万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440908
• 关键词: analytical ultracentrifugation; bioenergetics; biomedical equipment purchase; biomimetics; molecular assembly /self assembly; protein structure; stoichiometry

DESCRIPTION (provided by applicant): Nine investigators from three campuses of the University of Maryland seek funding for a Beckman Coulter Optima XL-I analytical ultracentrifuge. The user's group consists of Drs. Beckett (P1), Davis, Isaacs, Julin, Kahn, Munoz, and Greer from the Departments of Chemistry & Biochemistry and Chemical Engineering and the Center for Biological Structure & Organization at UMCP, Dr. David Weber from the Department of Biochemistry and Molecular Biology at the University of Maryland, Baltimore and Dr. Michael Summers from the HHMI and Department of Chemistry & Biochemistry, University of Maryland, Baltimore County. The centrifuge will be used for research in three general areas including analysis of macromolecular assembly in systems of biological interest, determination of the assembly properties of peptides and proteins for folding and high resolution structural studies, and characterization of the energetics and stoichiometries of synthetic biomimetic assemblies. Specific projects include determination of the energetics and stoichiometries of protein-protein interactions in transcriptional regulation and DNA recombination and repair, analysis of model ion channels, and determination of the assembly properties of model peptides for folding studies. The versatility provided by the absorption and interference optics of the Beckman Optima XL-I analytical ultracentrifuge will be essential to the success of the broad range of applications described in this proposal.

描述（由申请人提供）：来自三个校园的九名调查员 马里兰大学寻求贝克曼·库尔特（Beckman Coulter）Optima XL-I的资金 分析超速离心。用户的组由DRS组成。贝克特（P1）， 戴维斯，艾萨克斯，朱琳，卡恩，穆诺兹和格里尔化学部门 ＆生物化学与化学工程和生物结构中心 UMCP的组织，生物化学系的David Weber博士和 马里兰大学，巴尔的摩分子生物学和迈克尔博士 来自HHMI大学的夏季和化学与生物化学系 马里兰州，巴尔的摩县。离心机将用于三个研究 一般领域，包括分析大分子组装的系统 生物学兴趣，确定肽的装配特性和 用于折叠和高分辨率结构研究的蛋白质，以及 合成仿生的能量和石化的表征 集会。特定项目包括确定能量学和 转录调节中蛋白质 - 蛋白质相互作用的化学计量法 以及DNA重组和修复，模型离子通道的分析以及 确定模型肽的组装特性用于折叠研究。 通过吸收和干扰光学器件提供的多功能性 Beckman Optima XL-I分析性超级离心设备对于成功至关重要 在本提案中描述的广泛应用程序中。