ADVANCES IN BIOMEDICAL ANALYSIS BY TOF MASS SPECTROMETRY

描述：这项研究的主要目标是开发新的 对生物医学分析从业者有价值的技术和程序 基于飞行时间的特殊的和新兴的能力 质谱学(TOFMS)，特别是与电喷雾结合使用 电离(ESI)。由于最近的变化而产生的特殊品质 飞行时间质谱仪的发展是每秒产生超过50个质谱图 并实现了近100%的样品利用率 导致灵敏度可能超过质谱仪 只监测几个质量值。色谱学的意义 检测包括显著降低的检测下限和(通过 全光谱的附加数据)分析分辨率的提高 功率(对干扰的辨别力)至少10倍。一个系统 用于快速和灵敏地筛查滥用药物的体液 将这些功能与快速高效液相色谱一起开发 和电喷雾电离。此外，还提供了一种用于优化的系统 特定化合物的ESI条件将根据以下条件制定 这个实验室刚刚开发的预测模型。最后，串联式TOFMS 激光光解离和激光辅助仪器 碰撞诱导解离将用于合作的生物医学 旨在确定翻译后酰化的位置和 蛋白质脂蛋白中的脂肪酸周转率。它还将被用来 用电子能谱研究ESI形成的单电荷和多电荷离子 裂解质谱图也与ESI模型有关。

DESCRIPTION: The principal goal of this research is to develop new techniques and procedures of value to practitioners of biomedical analysis that are based on the special and emerging capabilities of time-of-flight mass spectrometry (TOFMS), especially in conjunction with electrospray ionization (ESI). The special qualities made possible by recent developments in TOFMS are the generation of over 50 mass spectra per second and the achievement of nearly 100 percent sample utilization efficiency leading to sensitivities that potentially exceed that of mass spectrometers monitoring only a few mass values. The implications for chromatographic detection include significantly lower detection limits and (through the additional data of the full spectrum) an increase in analytical resolving power (discrimination against interference) of at least 10 fold. A system for rapid and sensitive screening of bodily fluids for drugs of abuse will be developed using these capabilities along with fast liquid chromatography and electrospray ionization. In addition, a system for the optimization of conditions for the ESI of particular compounds will be developed based on a predictive model just developed in this laboratory. Finally, a tandem TOFMS instrument using both laser photodissociation and laser-assisted collision-induced dissociation will be used in a collaborative biomedical study aimed at determining the sites of post-translational acylation and the rate of fatty acid turnover in proteolipid protein. It will also be used to study the singly and multiply-charged ions formed by ESI by means of their fragmentation mass spectra relating these also to the ESI model.