DUAL TIME-OF-FLIGHT MASS SPECTROMETER: HIGH SPEED MS/MS

双飞行时间质谱仪：高速 MS/MS

• 批准号: 3303247
• 负责人: CHRISTIE G ENKE
• 金额: $ 27.58万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1993-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3303247
• 关键词: biomedical equipment development; gas chromatography mass spectrometry; lasers; mass spectrometry

We propose to develop a tandem time-of-flight (TOF) mass spectrometer which will have the capability to acquire MS/MS data at very high speed and sensitivity. The proposed instrument is expected to overcome many of the limitations of current MS/MS instrumentation; specifically the lengthy analysis time, the relatively large amount of sample required, and the problems associated with collisionally induced dissociation. The instrument is based on pulsed extraction of continuously formed ions, TOF separation of parent ions, photon fragmentation of selected parent ions, and TOF analysis of the daughter ions produced. The proposed instrument will produce up to one thousand daughter (or normal) spectra every second-about one hundred times more than current "fast" mass spectrometers. This instrument is unique in its highly efficient use of the sample. Time-of-flight instruments are widely known for their high on transmission and efficient extraction of ions from the source. The storage of ions formed between extractions makes very efficient use of a continuous-feed sample, and the ion packets produced have a signal-to-noise ratio that is up to one hundred times greater than the integration of an equivalent current for the same duration. The focusing of both the ions and the laser in the interaction region will lead to a very high fragmentation efficiency. Furthermore, the collection of all the daughter ions from each parent ion packet allows acquisition of daughter spectra with very little decrease in sensitivity over normal MS operation. The prototype instrument will be designed to obtain MS/MS spectra from samples introduced via a capillary gas chromatographic inlet. It will have at least unit mass resolution over a range of 0-1000 u for the daughter ions as well as for the parent ions, though the concept is extendable to much higher mass. Sample amounts required to produce complete MS/MS fragmentation maps should be comparable to or less than those needed for normal MS spectra on conventional gas chromatography/mass spectrometry instruments. These salient characteristics will be achieved by a novel combination of already-proven techniques. The proposed instrument should provide greatly enhanced capabilities in the many areas where MS/MS techniques are currently employed as well as opening up new areas of applications-particularly in high-performance chromatography MS/MS.

我们建议开发一种串联飞行时间（TOF）质谱仪， 将有能力以非常高的速度获取MS/MS数据， 灵敏度 预计拟议的文书将克服许多问题， 当前MS/MS仪器的局限性;特别是冗长的 分析时间，所需的相对大量的样品， 与碰撞诱导解离有关的问题。 该仪器基于连续形成的离子的脉冲提取， 母离子的TOF分离，所选母离子的光子碎裂 离子，并对所产生的子离子进行TOF分析。 拟议 仪器将产生多达一千个子（或正常）光谱 大约是目前“快”质量的一百倍 光谱仪 该仪器的独特之处在于其高效利用 示例. 飞行时间仪器以其高性能而广为人知。 传输和从源中有效提取离子。 存储 在提取之间形成的离子的浓度非常有效地利用了 连续进料样品，产生的离子包具有信噪比 这一比例高达一百倍以上的积分， 相同时间内的等效电流。 两种离子的聚焦 在相互作用区域的激光将导致非常高的 破碎效率 此外，收集所有的女儿 来自每个母离子包的离子允许获得子光谱 与正常MS操作相比，灵敏度几乎没有降低。 原型仪器将被设计为从以下物质获得MS/MS光谱： 通过毛细管气相色谱入口引入样品。 它将有 对于子体，在0-1000 u的范围内至少具有单位质量分辨率 离子以及母离子，尽管该概念可以扩展到 更高的质量。 生成完整MS/MS所需的样品量 碎片图应相当于或少于 常规气相色谱/质谱法的正态质谱 仪器. 这些突出的特点将通过一部小说来实现 结合已被证实的技术。 拟议的文书应 在MS/MS的许多领域提供大大增强的能力， 技术目前采用，以及开辟新的领域， 应用-特别是在高效色谱MS/MS中。