DUAL TIME-OF-FLIGHT MASS SPECTROMETER--HIGH SPEED MS/MS

拟议研究的目标是继续开发一种 一种可大幅提高质谱仪的串联质谱仪 可从样品组分获得的MS/MS数据量 从色谱柱洗脱。 当前MS/MS的缺点 在洗脱过程中收集多个质量扫描的仪器 一个单一的组成部分的时间已经取得了巨大的表征力量， MS/MS分析不适用于需要预先色谱分析的组分 分居 拟议的文书将具有重大意义， 在药物分析和其他生物研究中的适用性 它将提供独特和互补功能的应用程序 四极杆和扇形MS/MS仪器广泛用于现代 生物医学实验室 在不降低MS/MS数据速率的前提下， 在灵敏度要求放弃串联质量过滤器， 质量阵列分析仪 正在开发的仪器使用 具有离子存储器的连续电离， 连续进料样品，用离子镜进行脉冲离子提取， 分辨率离子聚束在相互作用区域，碎片的 通过光解离或表面-离子交换， 在相互作用区域的诱导解离，飞行时间分析 和时间阵列检测，以产生完整的 每个离子提取脉冲的产物离子谱。 500多个这样的 每秒钟都可以产生具有单位质量分辨率的光谱， 前体离子选择和产物离子检测。 将利用这一运作中的仪器探索相关的应用 在气相色谱MS/MS分析和持续的新仪器 液相色谱MS/MS和MALDI/MS/MS的发展。 此外，建议的PID过程的基础研究， 具有结构和生物学意义的分子将具有显著的 关于PID在各种各样的潜在应用的含义 MS/MS仪器

The goal of the proposed research is to continue the development of a tandem mass spectrometry (MS/MS) instrument capable of greatly increasing the amount of MS/MS data obtainable from sample components as they are eluting from a chromatographic column. The inability of current MS/MS instrumentation to collect more than a few mass scans during the elution time of a single component has made the great characterizing power of MS/MS analysis unavailable for components requiring prior chromatographic separation. The proposed instrument will have a significant applicability in pharmaceutical analysis and other bioresearch applications where it will offer unique and complementary capabilities to the quadupole and sector MS/MS instruments widely used in modern biomedical laboratories. The achievement of greatly increased MS/MS data rate without a reduction in sensitivity requires the abandonment of tandem mass filters in favor of a mass array analyzer. The instrument under development uses continuous ionization with ion storage for maximum utilization of continuous feed sample, pulsed ion extraction with an ion mirror for high resolution ion bunching at an interaction region, fragmentation of precursor ions of selected m/z value by photodissociation or surface- induced dissociation at the interaction region, time-of-flight analysis of the product ions, and time-array detection to produce a complete product ion spectrum for each ion extraction pulse. Over 500 such spectra can be produced each second with unit mass resolution for both precursor ion selection and product ion detection. The functioning instrument will be used to explore relevant applications in gas chromatography MS/MS analysis and continued new instrumentation developments in liquid chromatography MS/MS and MALDI/MS/MS. In addition, the proposed fundamental studies of the PID process on molecules of structural and biological interest will have significant implication regarding the potential application of PID in a wide variety of MS/MS instrumentation.