DUAL TIME-OF-FLIGHT MASS SPECTROMETER--HIGH SPEED MS/MS

拟议研究的目标是继续开发一种 可大幅提高的串联质谱仪 可从样本组件获取的MS/MS数据量 从色谱柱上洗脱出来。当前MS/MS的不能 在洗脱过程中收集大量扫描的仪器 单个组件的时间使其具有强大的表征能力 MS/MS分析不适用于需要事先进行层析的成分 分离。拟议的文书将具有重要的 在药物分析和其他生物研究中的适用性 它将提供独特和互补功能的应用程序 以四极杆和扇形MS/MS仪器广泛应用于现代 生物医学实验室。 实现了在不降低的情况下大大提高MS/MS数据速率 在灵敏度方面，需要放弃串联质量过滤器，转而支持 一台质量阵列分析器。正在开发的仪器使用 具有离子存储的连续电离，可最大限度地利用 连续进样，离子镜脉冲离子引出 在相互作用区域的分辨离子聚束，碎裂 选择的m/z值的前驱体离子通过光解或表面- 相互作用区的诱导解离，飞行时间分析 的产品离子，并对时间阵列进行检测，以产生完整的 每个离子提取脉冲的乘积离子光谱。超过500个这样的 每秒都可以产生具有单位质量分辨率的光谱 前体离子选择和产物离子检测。 发挥作用的仪器将用于探索相关应用 气相色谱中的MS/MS分析和不断发展的新仪器 液相色谱MS/MS和MALDI/MS/MS的研究进展 此外，还提出了对PID过程的基础研究 具有结构和生物学意义的分子将具有重要的 关于PID在多种领域的潜在应用的暗示 MS/MS仪器。

The goal of the proposed research is to continue the development of a tandem mass spectrometry (MS/MS) instrument capable of greatly increasing the amount of MS/MS data obtainable from sample components as they are eluting from a chromatographic column. The inability of current MS/MS instrumentation to collect more than a few mass scans during the elution time of a single component has made the great characterizing power of MS/MS analysis unavailable for components requiring prior chromatographic separation. The proposed instrument will have a significant applicability in pharmaceutical analysis and other bioresearch applications where it will offer unique and complementary capabilities to the quadupole and sector MS/MS instruments widely used in modern biomedical laboratories. The achievement of greatly increased MS/MS data rate without a reduction in sensitivity requires the abandonment of tandem mass filters in favor of a mass array analyzer. The instrument under development uses continuous ionization with ion storage for maximum utilization of continuous feed sample, pulsed ion extraction with an ion mirror for high resolution ion bunching at an interaction region, fragmentation of precursor ions of selected m/z value by photodissociation or surface- induced dissociation at the interaction region, time-of-flight analysis of the product ions, and time-array detection to produce a complete product ion spectrum for each ion extraction pulse. Over 500 such spectra can be produced each second with unit mass resolution for both precursor ion selection and product ion detection. The functioning instrument will be used to explore relevant applications in gas chromatography MS/MS analysis and continued new instrumentation developments in liquid chromatography MS/MS and MALDI/MS/MS. In addition, the proposed fundamental studies of the PID process on molecules of structural and biological interest will have significant implication regarding the potential application of PID in a wide variety of MS/MS instrumentation.