DUAL TIME-OF-FLIGHT MASS SPECTROMETER: HIGH SPEED MS/MS

我们建议开发一种串联式飞行时间(TOF)质谱仪 将能够以非常高的速度获取MS/MS数据，并且 敏感度。拟议的文书预计将克服许多 当前MS/MS仪器的局限性；特别是冗长 分析时间、所需样本量较大，以及 与碰撞诱导解离相关的问题。 该仪器基于对连续形成的离子的脉冲提取， TOF分离母体离子，选定母体的光子碎裂 离子，并对产生的子离子进行TOF分析。建议数 仪器将产生多达1000个子谱(或正常谱) 每秒--大约是目前“快”质量的一百倍 分光计。这台仪器的独特之处在于它能高效地使用 样本。飞行时间仪器以其高性能广为人知 从源头传输和有效地提取离子。存储空间 在萃取之间形成的离子非常有效地利用了 连续进样，产生的离子包具有信噪比 这一比率最高可比 相同持续时间内的等值电流。两种离子的聚焦 而相互作用区中的激光将导致非常高的 碎裂效率。此外，所有女儿的收藏 来自每个父离子包的离子允许获取子谱 与正常的MS操作相比，灵敏度几乎没有下降。 原型仪器将设计为从以下位置获取MS/MS光谱 样品通过毛细管气相色谱进样口进样。它将会有 女儿的单位质量分辨率至少在0-1000u范围内 离子和母离子，尽管这个概念可以扩展到 大得多的质量。生产完整的MS/MS所需的样本量 碎片图应与以下所需的图相当或更少 常规气相色谱/质谱仪的正常质谱学研究 乐器。这些显著的特征将通过一部小说来实现 已被证实的技术的结合。拟议的文书应 在MS/MS的多个领域提供极大增强的功能 目前正在使用的技术以及开辟了新的领域 应用-特别是在高效色谱MS/MS中的应用。

We propose to develop a tandem time-of-flight (TOF) mass spectrometer which will have the capability to acquire MS/MS data at very high speed and sensitivity. The proposed instrument is expected to overcome many of the limitations of current MS/MS instrumentation; specifically the lengthy analysis time, the relatively large amount of sample required, and the problems associated with collisionally induced dissociation. The instrument is based on pulsed extraction of continuously formed ions, TOF separation of parent ions, photon fragmentation of selected parent ions, and TOF analysis of the daughter ions produced. The proposed instrument will produce up to one thousand daughter (or normal) spectra every second-about one hundred times more than current "fast" mass spectrometers. This instrument is unique in its highly efficient use of the sample. Time-of-flight instruments are widely known for their high on transmission and efficient extraction of ions from the source. The storage of ions formed between extractions makes very efficient use of a continuous-feed sample, and the ion packets produced have a signal-to-noise ratio that is up to one hundred times greater than the integration of an equivalent current for the same duration. The focusing of both the ions and the laser in the interaction region will lead to a very high fragmentation efficiency. Furthermore, the collection of all the daughter ions from each parent ion packet allows acquisition of daughter spectra with very little decrease in sensitivity over normal MS operation. The prototype instrument will be designed to obtain MS/MS spectra from samples introduced via a capillary gas chromatographic inlet. It will have at least unit mass resolution over a range of 0-1000 u for the daughter ions as well as for the parent ions, though the concept is extendable to much higher mass. Sample amounts required to produce complete MS/MS fragmentation maps should be comparable to or less than those needed for normal MS spectra on conventional gas chromatography/mass spectrometry instruments. These salient characteristics will be achieved by a novel combination of already-proven techniques. The proposed instrument should provide greatly enhanced capabilities in the many areas where MS/MS techniques are currently employed as well as opening up new areas of applications-particularly in high-performance chromatography MS/MS.