BIOLOGY OF SIV IN THE AFRICAN GREEN MONKEY

非洲绿猴体内 SIV 的生物学

• 批准号: 2797158
• 负责人: CHRISTOPHER James MILLER
• 金额: $ 2.34万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-30 至 1999-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2797158
• 关键词: Cercopithecidae; cellular immunity; host organism interaction; humoral immunity; mature animal; newborn animals; simian immunodeficiency virus; virus infection mechanism

DESCRIPTION: (Adapted from Applicant's Abstract). This RO3 is an application to the Fogarty International Center AIDS International Research and Training Program. The objective of this collaborative study between California Regional Primate Research Center (CRPRC) and the Institute of Primate Research (IPR) in Kenya is to define the biology of simian immunodeficiency virus (SIVagm) in a natural host, the African green monkey (AGM), Cercopethecus aethiops. It is noteworthy that the AGMs have not been heavily used in AIDS and SIV research. Despite the fact that large numbers of African green monkeys (AGMs) in the wild and captivity are persistently infected with SIVagm, they do not display clinical signs of simian AIDS, unlike Asian macaques infected with SIVagm. This observation has led to suggestions that SIVagm and its natural host have co-evolved into a nonpathogenic relationship. This study will therefore seek to clarify the biology of SIVagm in the AGM by defining (a) the biology of SIVagm in naturally infected AGMs from the wild, (b) the biology of SIVagm in experimentally infected captive AGMs, (c) the role of routes of virus inoculation on SIVagm pathogenesis and (d) the biology of SIVagm on immunologically immature host, the AGM newborn. The authors suggest that AGMs provide an excellent model to seek answers to these questions, because (i) over one third of wild and captive AGMs are SIV-infected (ii) AGM-infection with SIVagm is nonpathogenic and (iii) large numbers of AGMs are available for the proposed study. This study therefore proposes to use naturally infected AGMs, intravenously or mucosally infected adult AGMs and newborn AGMs to examine and define (a) anti-SIV cytotoxic T-cell lympocyte (CTL) and antibody responses, (b) viral burden in the peripheral blood and plasma, (c) the SIVagm distribution in tissues and organs.

描述：（改编自申请人摘要）。 RO3是一个 福格蒂国际艾滋病研究中心（Fogarty International Center AIDS International Research） 和培训计划。 这项合作研究的目的是， 加州地区灵长类动物研究中心（CRPRC）和研究所 肯尼亚的灵长类动物研究（IPR）将定义猿类的生物学特征， 免疫缺陷病毒（SIVagm）在一个自然宿主，非洲绿色猴 (AGM)、Cercopethecus aethiops. 值得注意的是，AGM没有被 大量用于艾滋病和SIV研究。 尽管大量的 野生和圈养的非洲绿色猴（AGM）的数量持续下降， 感染SIVagm后，它们不会表现出猿类艾滋病的临床症状， 与感染SIVagm的亚洲猕猴不同。 这一观察结果导致 这表明SIVagm和它的自然宿主已经共同进化成了一种 非致病关系。 因此，本研究将力求澄清 在年度大会上定义（a）SIVagm的生物学， （B）来自野生的天然感染的AGM， 实验感染的圈养AGM，（c）病毒途径的作用 （d）SIVagm的生物学特性， 免疫不成熟的宿主AGM新生儿 作者认为 年度股东大会为寻求这些问题的答案提供了一个很好的模式，因为 (i)超过三分之一的野生和圈养AGM感染SIV（ii） AGM感染SIVagm是非致病性的，（iii）大量AGM 可用于拟议的研究。 因此，本研究建议使用 天然感染的AGM，静脉内或粘膜感染的成人AGM， 新生儿AGM检查和定义（a）抗SIV细胞毒性T细胞巨噬细胞 (CTL)和抗体应答，（B）外周血中的病毒负荷， （c）SIVagm在组织和器官中的分布。