BETA-TUBLIN AS A PROBE OF MICROTUBULE MORPHOGENESIS

β-微管蛋白作为微管形态发生的探针

• 批准号: 2459589
• 负责人: FRANK E SOLOMON
• 金额: $ 28.73万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2459589
• 关键词: SDS polyacrylamide gel electrophoresis; Saccharomyces; binding proteins; cell cycle; cell cycle proteins; gene expression; gene interaction; histogenesis; laboratory rabbit; microtubules; oligonucleotides; protein biosynthesis; protein folding; protein sequence; protein structure function; radionuclides; tissue /cell culture; tubulin; western blottings

DESCRIPTION: The molecular mechanisms of microtubule morphogenesis and their regulation are addressed by this application. Specifically, this proposal focuses on Rbl2p, a S. cerevisiae protein involved at some basic level in the pathway from tubulin gene-products to functional microtubules. Rbl2p was identified by this group by its ability to suppress the deleterious effects of overexpression of b-tubulin. It is similar in sequence to murine cofactor A which has also been implicated in microtubule assembly. Other gene-products related to microtubule assembly and/or function will also be examined.

描述：微管形态发生的分子机制， 它们的调节由本申请解决。 具体来说， Rbl 2 p是一个S.酿酒蛋白参与了一些基本的 在微管蛋白基因产物到功能性微管的通路中的水平。 Rbl 2 p被这个小组通过其抑制 b-微管蛋白过度表达的有害作用。 它类似于 小鼠辅因子A的序列，该序列也与微管相关， 组装件. 其他与微管组装相关的基因产物和/或 功能也将被检查。