SOLUBLE AND ANTIGENIC PHOSPHOINOSITIDE PHOSPHATES

可溶性和抗原性磷酸肌醇磷酸盐

基本信息

批准号：
2643516
负责人：
GLENN DOWNES PRESTWICH
金额：
$ 9.18万
依托单位：
ECHELON BIOSCIENCES, INC.
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
起止时间：
1998-05-01 至 1999-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2643516
关键词：
chemical synthesis method development phosphatidate phosphatase phosphatidylinositols

项目摘要

DESCRIPTION (Adapted from the application): Phosphoinositide polyphosphates (PIPns) are key signaling molecules in cellular communication via protein kinases, in endo- and exocytosis, and in vesicular trafficking of proteins. The PIPns consist of a family of seven molecular scaffolds based on the number and position of phosphates on the inositol head group. Most PIPns are available only by total chemical synthesis, requiring that biologists in over 200 labs worldwide locate and academic collaborator. The demand of quantities of PIPns with a variety of diacylglyceryl chains now exceeds the capacity of these labs. Availability of these signaling molecules and reagents for their detection will facilitate drug discovery in allergy, inflammation, apoptosis, cancer and neurological diseases. Echelon Research Laboratories (ERL) is a three-tier business strategy. First, ERL will develop PIPn synthesis for four different diacylglycerol derivatives of molecular scaffolds to make "matched sets" of PIPn derivatives, including short-chain, intermediate chain, and functionalize "tetherable" analogs. They will use functionalized PIPns to prepare antigenic forms of the PIPns for eliciting PIPn-specific antisera. Second, they will develop reagent kits for enzyme and binding protein assays. Third, they will use these reagents and assay methods in drug discovery. PROPOSED COMMERCIAL APPLICATION: Potential commercial application not available.

描述（根据应用程序改编）：磷酸肌醇多磷酸盐（PIPN）是细胞中的关键信号分子通过蛋白激酶，内胞胞病和胞吐作用以及在蛋白质的囊泡运输。 pipns由一个七口之家组成分子支架基于磷酸盐的数量和位置肌醇头组。大多数PIPN仅可通过总化学品获得综合，要求全球200多个实验室的生物学家定位和学术合作者。各种PIPN的需求二酰基聚糖链现在超过了这些实验室的容量。可用性这些信号分子和试剂的检测促进过敏，炎症，凋亡，癌症和神经疾病。梯队研究实验室（ERL）是三层业务策略。首先，ERL将开发四个的PIPN合成分子支架的不同二酰基甘油衍生物使 PIPN导数的“匹配集”，包括短链，中间链，并功能化“可连接”类似物。他们会使用功能化的PIPN以制备抗原形式的PIPN用于引发 PIPN特异性抗血清。其次，他们将开发用于酶的试剂盒和结合蛋白测定。第三，他们将使用这些试剂和测定药物发现的方法。拟议的商业应用：潜在的商业应用不可用。