REPRESSOR AND ANTIREPRESSOR PROTEINS OF BACTERIOPHAGE

这项研究计划的目标是了解序列和 在涉及蛋白质的组装过程中，结构决定了特异性 DNA和蛋白质之间的相互作用。DNA结合蛋白是至关重要的 许多细胞和病毒过程的参与者及其生物学 活性通常由其他蛋白质的结合来调节， 通过共价修饰和/或通过降解。在其中的每一个中 在病例中，特异性是至关重要的。DNA结合蛋白必须能够 在存在大量过剩的情况下识别正确的位置 非特定DNA和调节性修饰或结合事件必须 定位于细胞中的一个或几个关键蛋白质。了解这些 过程是分子和结构基础研究的一个重要目标 生物学，在医学、生物技术和生物科学方面具有潜在的应用 设计新的蛋白质和调节电路。其中一个长期的 我们研究计划的目的是了解弧形抑制因子是如何 噬菌体P22是一种带状-螺旋-螺旋转录因子，与操纵子DNA结合 并调节转录。其他目的是了解底物 细菌C-末端特异性的TSP和CLP家族的识别 并确定了单链重组酶标记的机理和规律。 介导C端肽的共翻译加成的系统 到蛋白质。在这些系统中，我们每周都要了解 序列和结构因素决定了结合的特异性。何以 区分运算符和非运算符的转录因子 DNA？细胞内的蛋白水解酶如何选择目标底物？什么 确定共翻译标记哪些细胞内蛋白质 SSRA系统？转录因子、蛋白分解和多肽 标记也有生物上的联系；DNA结合蛋白通常 C-末端特异性蛋白水解酶的靶标和单链重组酶A系统标记 被这些酶降解的蛋白质。

The objective of this research program is to understand how sequence and structure determine specificity in assembly processes involving protein- DNA and protein-protein interactions. DNA-binding proteins are critical participants in many cellular and viral processes and their biological activities are frequently regulated by the binding of other proteins, by covalent modification, and/or by degradation. In each of these cases, specificity is critical. DNA-binding proteins must be capable of recognizing the correct sites in the presence of a vast excess of nonspecific DNA and regulatory modifications or binding events must be targeted to one or a few key proteins the cell. Understanding these processes is a key goal of basic research in molecular and structural biology, with potential applications in medicine, biotechnology, and the design of novel proteins and regulatory circuits. One of the long-term aims of our research program is to understand how the Arc repressor of phage P22, a ribbon-helix-helix transcription factor, binds operator DNA and regulates transcription. Other aims are to understand substrate recognition by the Tsp and Clp families of bacterial C-terminal specific proteases and to determine the mechanism and rules of the ssrA tagging system which mediates cotranslational addition of C-terminal peptides to proteins. In each of these systems, we week to understand how sequence and structural factors determine binding specificity. How does a transcription factor discriminate between operator and non-operator DNA? How do intracellular proteases select target substrates? What determines which intracellular proteins are cotranslationally tagged by the ssrA system? Transcription factors, proteolysis, and peptide tagging are also connected biologically; DNA-binding proteins are often the targets of C-terminal specific proteases and the ssrA system marks proteins for degradation by these enzymes.