REPRESSOR AND ANTIREPRESSOR PROTEINS OF BACTERIOPHAGE

这项研究计划的目的是了解如何序列和 结构决定蛋白质组装过程特异性， DNA和蛋白质-蛋白质相互作用DNA结合蛋白是关键 参与许多细胞和病毒过程， 活性经常受到其它蛋白质的结合的调节， 通过共价修饰和/或通过降解。 在这些 在某些情况下，特异性至关重要。 DNA结合蛋白必须能够 识别正确的位置， 非特异性DNA和调节性修饰或结合事件必须 靶向细胞中的一种或几种关键蛋白质。 了解这些 过程是分子和结构领域基础研究的一个关键目标 生物学，在医学，生物技术和生物技术方面具有潜在的应用， 设计新的蛋白质和调控电路。 一个长期的 我们的研究计划的目的是了解Arc阻遏物是如何 噬菌体P22是一种带-螺旋-螺旋转录因子，可与操纵基因DNA结合 并调节转录。 其他目的是了解基质 Tsp和Clp家族对细菌C-末端特异性 蛋白酶，并确定ssrA标记的机制和规则 介导C-末端肽的共翻译添加的系统 到蛋白质。 在这些系统中，我们每周都要了解 序列和结构因素决定结合特异性。 如何 一个转录因子区分操纵基因和非操纵基因 DNA？ 细胞内蛋白酶如何选择靶底物？ 什么 确定哪些细胞内蛋白质被标记， ssrA系统 转录因子、蛋白水解和肽 标记也与生物学有关; DNA结合蛋白通常 C-末端特异性蛋白酶的靶标和ssrA系统标记 蛋白质被这些酶降解。

The objective of this research program is to understand how sequence and structure determine specificity in assembly processes involving protein- DNA and protein-protein interactions. DNA-binding proteins are critical participants in many cellular and viral processes and their biological activities are frequently regulated by the binding of other proteins, by covalent modification, and/or by degradation. In each of these cases, specificity is critical. DNA-binding proteins must be capable of recognizing the correct sites in the presence of a vast excess of nonspecific DNA and regulatory modifications or binding events must be targeted to one or a few key proteins the cell. Understanding these processes is a key goal of basic research in molecular and structural biology, with potential applications in medicine, biotechnology, and the design of novel proteins and regulatory circuits. One of the long-term aims of our research program is to understand how the Arc repressor of phage P22, a ribbon-helix-helix transcription factor, binds operator DNA and regulates transcription. Other aims are to understand substrate recognition by the Tsp and Clp families of bacterial C-terminal specific proteases and to determine the mechanism and rules of the ssrA tagging system which mediates cotranslational addition of C-terminal peptides to proteins. In each of these systems, we week to understand how sequence and structural factors determine binding specificity. How does a transcription factor discriminate between operator and non-operator DNA? How do intracellular proteases select target substrates? What determines which intracellular proteins are cotranslationally tagged by the ssrA system? Transcription factors, proteolysis, and peptide tagging are also connected biologically; DNA-binding proteins are often the targets of C-terminal specific proteases and the ssrA system marks proteins for degradation by these enzymes.