PEPTIDE MODELS OF TWO HELICAL REGIONS IN GP41 ECTODOMAIN

GP41胞外域中两个螺旋区域的肽模型

• 批准号: 2667713
• 负责人: THOMAS J MATTHEWS
• 金额: $ 29.62万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-30 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2667713
• 关键词: HIV envelope protein gp41; gene mutation; human immunodeficiency virus 1; molecular cloning; protein structure function; recombinant proteins; site directed mutagenesis; virus infection mechanism

DESCRIPTION (Adapted from investigator's abstract): The synthetic peptides, DP107 and DP178, derived from gp41 ectodomain, have been shown to inhibit HIV-transition to a coiled-coil structure; suggesting that the corresponding region of gp41 undergoes structural rearrangement as an obligate step in membrane fusion. The second peptide, DP178 blocks HIV-1 mediated cell fusion at much lower concentration. The application focuses on the mechanism of action of DP178 by deriving virus "escape" mutants, and mapping the DP178 interactive sites. Correlations between the binding characteristics and the phenotypic changes of the escape mutants would support the critical role of these interactions in virus life cycle.

描述(改编自调查人员摘要)：合成的 来源于gp41胞外结构域的多肽DP107和DP178已被证明 抑制HIV向螺旋线圈结构的转变；这表明 Gp41的相应区域经历了结构重排 膜融合的必备步骤。第二个多肽DP178可阻断HIV-1 以低得多的浓度介导细胞融合。应用程序 重点阐述了DP178通过衍生病毒“逃逸”的作用机制 突变体，并绘制DP178交互站点的地图。两者之间的相关性 脱氧核糖核酸的结合特性和表型变化 突变体将支持这些相互作用在病毒中的关键作用 生命周期。