MAGNESIUM SULFATE EFFECTS ON PREGNANT HUMAN UTERUS

硫酸镁对怀孕人类子宫的影响

• 批准号: 2669661
• 负责人: WILLIAM W HURD
• 金额: $ 22.3万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2669661
• 关键词: calcium ion; clinical research; drug interactions; enzyme activity; enzyme inhibitors; human pregnant subject; human tissue; magnesium; muscle contraction; myometrium; myosin light chain kinase; phospholipase C; pregnancy; protein kinase C; sulfates; tissue /cell culture; tocolytic agents; uterus

DESCRIPTION (Adapted from the Investigator's Abstract): The hypothesis of this proposal is that MgS04 decrease myometrial contractility by interfering with the stimulatory effect of Ca2+ at intracellular locations. To test this hypothesis, the effects of MgS04 on specific steps in the contractile response of human myometrium will be evaluate; these studies will include: 1) simultaneously evaluating contractile force and cytosolic Ca2+ to determine the relationship between the ability of MgS04 to decrease contractility and to inhibit increases in cytosolic Ca2+; 2) determining if MgS04 inhibits activation of phospholipase C; 3) determining if MgS04 inhibits agonist-induced activation of protein kinase C; and 4) determining if MgS04 inhibits activation of myosin light chain kinase. For these studies strips of myometrium and cultured uterine myocytes will be obtained from pregnant women undergoing cesarean section deliveries, which are uncomplicated by infection or pharmacologic treatment for premature labor.

描述（改编自研究者摘要）：假设