MAGNESIUM SULFATE EFFECTS ON PREGNANT HUMAN UTERUS

硫酸镁对怀孕人类子宫的影响

• 批准号: 2889537
• 负责人: WILLIAM W HURD
• 金额: $ 21.65万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2889537
• 关键词: calcium ion; clinical research; drug interactions; enzyme activity; enzyme inhibitors; human pregnant subject; human tissue; magnesium; muscle contraction; myometrium; myosin light chain kinase; phospholipase C; pregnancy; protein kinase C; sulfates; tissue /cell culture; tocolytic agents; uterus

DESCRIPTION (Adapted from the Investigator's Abstract): The hypothesis of this proposal is that MgS04 decrease myometrial contractility by interfering with the stimulatory effect of Ca2+ at intracellular locations. To test this hypothesis, the effects of MgS04 on specific steps in the contractile response of human myometrium will be evaluate; these studies will include: 1) simultaneously evaluating contractile force and cytosolic Ca2+ to determine the relationship between the ability of MgS04 to decrease contractility and to inhibit increases in cytosolic Ca2+; 2) determining if MgS04 inhibits activation of phospholipase C; 3) determining if MgS04 inhibits agonist-induced activation of protein kinase C; and 4) determining if MgS04 inhibits activation of myosin light chain kinase. For these studies strips of myometrium and cultured uterine myocytes will be obtained from pregnant women undergoing cesarean section deliveries, which are uncomplicated by infection or pharmacologic treatment for premature labor.

描述(改编自《调查者摘要》)：假设 这一建议是，镁04通过以下方式降低子宫肌层收缩能力 干扰细胞内钙离子的刺激作用 地点。为了验证这一假设，MGS04对特定步骤的影响 在人类子宫肌层的收缩反应中将被评估；这些 研究将包括：1)同时评估收缩力量和 胞内Ca~(2+)与测定MgS_4能力的关系 降低细胞收缩能力，抑制细胞内钙离子升高；2) 确定MgS04是否抑制磷脂酶C的激活；3) 测定MgS04是否抑制激动剂诱导的蛋白激酶激活 C；以及4)确定MgS04是否抑制肌球蛋白轻链的激活 激活剂。在这些研究中，子宫肌层和培养的子宫 心肌细胞将从剖宫产孕妇身上获得 分娩，不会因感染或药物原因而复杂化 治疗早产。