MAGNESIUM SULFATE EFFECTS ON PREGNANT HUMAN UTERUS

硫酸镁对怀孕人类子宫的影响

• 批准号: 6181761
• 负责人: WILLIAM W HURD
• 金额: $ 1.76万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6181761
• 关键词: calcium ion; clinical research; drug interactions; enzyme activity; enzyme inhibitors; human pregnant subject; human tissue; magnesium; muscle contraction; myometrium; myosin light chain kinase; phospholipase C; pregnancy; protein kinase C; sulfates; tissue /cell culture; tocolytic agents; uterus

DESCRIPTION (Adapted from the Investigator's Abstract): The hypothesis of this proposal is that MgS04 decrease myometrial contractility by interfering with the stimulatory effect of Ca2+ at intracellular locations. To test this hypothesis, the effects of MgS04 on specific steps in the contractile response of human myometrium will be evaluate; these studies will include: 1) simultaneously evaluating contractile force and cytosolic Ca2+ to determine the relationship between the ability of MgS04 to decrease contractility and to inhibit increases in cytosolic Ca2+; 2) determining if MgS04 inhibits activation of phospholipase C; 3) determining if MgS04 inhibits agonist-induced activation of protein kinase C; and 4) determining if MgS04 inhibits activation of myosin light chain kinase. For these studies strips of myometrium and cultured uterine myocytes will be obtained from pregnant women undergoing cesarean section deliveries, which are uncomplicated by infection or pharmacologic treatment for premature labor.

描述（改编自研究者摘要）： 该建议是MgSO 4通过以下方式降低子宫肌层收缩性 干扰细胞内Ca 2+的刺激作用 地点为了测试该假设，研究了MgS 〇 4对特定步骤的影响。 将评估人子宫肌层收缩反应的影响;这些 研究将包括：1）同时评估收缩力， 胞浆Ca 2+测定能力与MgSO 4 降低收缩性并抑制胞浆Ca 2+的增加; 2） 确定MgS 〇 4是否抑制磷脂酶C的活化; 3） 确定MgS 〇 4是否抑制激动剂诱导的蛋白激酶活化 和4）确定MgS 〇 4是否抑制肌球蛋白轻链的活化 激酶。在这些研究中，子宫肌层和培养的子宫内膜 肌细胞将从进行剖腹产的孕妇获得 没有感染或药物并发症的分娩 早产的治疗