METABOLIC AND HORMONAL CONTROL OF CARDIAC CONTRACTION
METABOLIC AND HORMONAL CONTROL OF CARDIAC CONTRACTION
批准号:
2859335
负责人:
Gary Brooker
金额:
$27.08万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1981
资助国家:
美国
项目状态:
已结题
起止时间:
1981-09-01 至 2000-04-30
中文摘要
描述(改编自申请人的摘要):研究是
英文摘要
DESCRIPTION (adapted from the applicant's abstract): Studies are
proposed to unravel the intracellular mechanisms involved in the short
and long term regulation by Ca2+ of cAMP accumulation in C6-2B rat
glioma cells. During the previous grant period the applicant
demonstrated that Ca2+ exerts a dramatic inhibitory action on cAMP
accumulation and upon adenylyl cyclase in C6-213 cells. These cells are
a useful model to study Ca2+ inhibition of adenylyl cyclase, since they
almost exclusively contain type VI adenylyl cyclase, a recently cloned
form which is inhibited by submicromolar concentrations of Ca2+. The
applicant has proposed that Ca2+ plays an important role in the short
and long term regulation of the concentration of cAMP in C6-2B cells.
The first focus will be upon the mechanism by which Ca2+ regulates cAMP
metabolism. Later in the project the applicant will extend these studies
to ascertain the involvement of Ca2+ in hormone and/or cAMP induced
refractoriness. Methodology based upon the applicant's previous work and
that of others, is proposed for the high resolution simultaneous high
speed ratio imaging of Ca2+ and cAMP in living cells at multiple planes
of focus using convolution-deconvolution techniques to improve spatial
image resolution based upon knowledge of the microscopic point spread
function. Thus, detailed three dimensional intracellular time-
concentration information about the interdependent kinetics of both
second messengers will help the applicant to examine more localized
second messenger changes which might not be detected by standard imaging
techniques. The concentrations of both cAMP and Ca2+ will be
experimentally modified to examine the short and long term
interdependency between the two in terms of their steady state
concentration and oscillatory behavior. Studies will involve
pharmacological manipulation of Ca2+ and cAMP, direct microinjection of
the second messengers or their modifiers or analogs, or modification of
cAMP synthesis with antisense RNA complementary to type VI adenylyl
cyclase, the major form of adenylyl cyclase in C6-2B cells and abundant
in cardiac tissue. Further studies are aimed at defining this
mechanism, at the level of adenylyl cyclase or on G protein-cyclase
interactions, by examining the mechanism of Ca2+ inhibition of adenylyl
cyclase in reconstituted preparations of G proteins and cyclase in
phospholipid vesicles.
期刊论文(19)
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2-Aminopurine inhibits RNA and protein synthesis and reduces catecholamine desensitization in C6-2B rat glioma cells.
2-Aminopurine 抑制 C6-2B 大鼠神经胶质瘤细胞中的 RNA 和蛋白质合成并减少儿茶酚胺脱敏。
DOI:
10.1016/0014-5793(92)80415-d
发表时间:
1992
期刊:
FEBS letters
影响因子:
3.5
作者:
[DeBernardi,MA, Brooker,G]
通讯作者:
Brooker,G
Forskolin-stimulated cyclic AMP accumulation mediates protein synthesis-dependent refractoriness in C6-2B rat glioma cells.
Forskolin 刺激的环 AMP 积累介导 C6-2B 大鼠神经胶质瘤细胞中蛋白质合成依赖性不应性。
DOI:
--
发表时间:
1983
期刊:
Journal of cyclic nucleotide and protein phosphorylation research
影响因子:
--
作者:
[Barovsky,K, Pedone,C, Brooker,G]
通讯作者:
Brooker,G
Ca2+ inhibition of beta-adrenergic receptor- and forskolin-stimulated cAMP accumulation in C6-2B rat glioma cells is independent of protein kinase C.
Ca2 对 C6-2B 大鼠神经胶质瘤细胞中 β-肾上腺素能受体和毛喉素刺激的 cAMP 积累的抑制与蛋白激酶 C 无关。
DOI:
--
发表时间:
1993
期刊:
Molecular pharmacology
影响因子:
3.6
作者:
[Debernardi,MA, Munshi,R, Brooker,G]
通讯作者:
Brooker,G
P2U-purinergic receptors on C6-2B rat glioma cells: modulation of cytosolic Ca2+ and cAMP levels by protein kinase C.
C6-2B 大鼠神经胶质瘤细胞上的 P2U 嘌呤能受体:蛋白激酶 C 调节胞质 Ca2 和 cAMP 水平。
DOI:
--
发表时间:
1993
期刊:
Molecular pharmacology
影响因子:
3.6
作者:
[Munshi,R, DeBernardi,MA, Brooker,G]
通讯作者:
Brooker,G
Selective reduction of forskolin-stimulated cyclic AMP accumulation by inhibitors of protein synthesis.
通过蛋白质合成抑制剂选择性减少毛喉素刺激的环 AMP 积累。
DOI:
10.1126/science.6190226
发表时间:
1983
期刊:
Science (New York, N.Y.)
影响因子:
--
作者:
[Brooker,G, Pedone,C, Barovsky,K]
通讯作者:
Barovsky,K
共 14 条
Super-Res Holographic Microscopy to Advance Research on Golgi Apparatus Function
-
批准号:9085327
-
项目类别:
-
资助金额:$40.54万
-
财政年份:2013
-
负责人:Gary Brooker
-
依托单位:
Super-Res Holographic Microscopy to Advance Research on Golgi Apparatus Function
-
批准号:9326590
-
项目类别:
-
资助金额:$24.75万
-
财政年份:2013
-
负责人:Gary Brooker
-
依托单位:
Super-Res Holographic Microscopy to Advance Research on Golgi Apparatus Function
-
批准号:8710283
-
项目类别:
-
资助金额:$31.44万
-
财政年份:2013
-
负责人:Gary Brooker
-
依托单位:
Super-Res Holographic Microscopy to Advance Research on Golgi Apparatus Function
-
批准号:8488631
-
项目类别:
-
资助金额:$39.68万
-
财政年份:2013
-
负责人:Gary Brooker
-
依托单位:
Development of a Motionless 3D Fluorescence Microscope
-
批准号:7611265
-
项目类别:
-
资助金额:$14.42万
-
财政年份:2009
-
负责人:Gary Brooker
-
依托单位:
Development of a Motionless 3D Fluorescent Microscope
-
批准号:8648830
-
项目类别:
-
资助金额:$81.45万
-
财政年份:2008
-
负责人:Gary Brooker
-
依托单位:
METABOLIC AND HORMONAL CONTROL OF CARDIAC CONTRACTION
-
批准号:3340147
-
项目类别:
-
资助金额:$28.86万
-
财政年份:1981
-
负责人:Gary Brooker
-
依托单位:
METABOLIC AND HORMONAL CONTROL OF CARDIAC CONTRACTION
-
批准号:3340151
-
项目类别:
-
资助金额:$22.26万
-
财政年份:1981
-
负责人:Gary Brooker
-
依托单位:
METABOLIC AND HORMONAL CONTROL OF CARDIAC CONTRACTION
-
批准号:3340148
-
项目类别:
-
资助金额:$21.3万
-
财政年份:1981
-
负责人:Gary Brooker
-
依托单位:
METABOLIC AND HORMONAL CONTROL OF CARDIAC CONTRACTION
-
批准号:3340149
-
项目类别:
-
资助金额:$18.75万
-
财政年份:1981
-
负责人:Gary Brooker
-
依托单位:
METABOLIC AND HORMONAL CONTROL OF CARDIAC CONTRACTION
-
批准号:2445105
-
项目类别:
-
资助金额:$5.84万
-
财政年份:1981
-
负责人:Gary Brooker
-
依托单位:
METABOLIC AND HORMONAL CONTROL OF CARDIAC CONTRACTION
-
批准号:2028100
-
项目类别:
-
资助金额:$19.88万
-
财政年份:1981
-
负责人:Gary Brooker
-
依托单位:
METABOLIC AND HORMONAL CONTROL OF CARDIAC CONTRACTION
-
批准号:3340143
-
项目类别:
-
资助金额:$19.45万
-
财政年份:1981
-
负责人:Gary Brooker
-
依托单位:
METABOLIC AND HORMONAL CONTROL OF CARDIAC CONTRACTION
-
批准号:2216366
-
项目类别:
-
资助金额:$24.73万
-
财政年份:1981
-
负责人:Gary Brooker
-
依托单位:
METABOLIC AND HORMONAL CONTROL OF CARDIAC CONTRACTION
-
批准号:3340150
-
项目类别:
-
资助金额:$21.3万
-
财政年份:1981
-
负责人:Gary Brooker
-
依托单位:
海外基金