权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Super-Res Holographic Microscopy to Advance Research on Golgi Apparatus Function

超分辨率全息显微镜推进高尔基体功能研究

基本信息

批准号：
9326590
负责人：
Gary Brooker
金额：
$ 24.75万
依托单位：
UNIV OF ARKANSAS FOR MED SCIS
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-08-01 至 2018-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9326590
关键词：
3-Dimensional Affect Aging Algorithms Antibodies Arkansas Back Boxing Caliber Cells Chlamydia Collection Color Complex Computer software Data Defect Dimensions Electron Microscopy Fluorescence Fluorescence Microscopy Funding Glaucoma Golgi Apparatus Grant Health Hela Cells Holography Human Image Individual Laboratories Life Light Light Microscope Lighting Manuscripts Measures Medical Membrane Membrane Protein Traffic Membrane Proteins Methods Microscope Microscopic Microscopy Microtubules National Institute of General Medical Sciences Needles Neurodegenerative Disorders Organelles Pathway interactions Performance Preparation Property Proteins Publications Research Research Personnel Resolution Role Science Series Small Business Innovation Research Grant Source Staging Structure System Techniques Technology Time United States National Institutes of Health Universities Virus Diseases Vision Work base cell fixing density digital fluorescence microscope implementation research improved in vivo innovation light microscopy live cell imaging meter nanometer novel strategies protein complex protein distribution protein function prototype reconstruction three dimensional structure

项目摘要

Three of the investigators on this application (Storrie at the University of Arkansas for Medical Sciences, Brooker and Rosen at Johns Hopkins University and Ben Gurion University) have been collaborating for the last 7 years on the vision that application of holography to fluorescence microscopy could simplify 3D microscopy and also capitalize on the super-resolution capabilities afforded by holography. This work, supported by NSF, NIH SBIR, and NIST grants hasnow yielded a 3-dimensional, fluorescence microscopic technique with super-resolving properties. It is based upon a new holography concept called FINCH, developed by two of the investigators (Rosen and Brooker). FINCH is a holographic technique in which the incoherent fluorescence microscope emission is imaged with a simple setup consisting of a spatial light modulator inserted into the light path of a standard fluorescence microscope stand, an attached digital camera, and a non-laser, externally shuttered light source. Recently the FINCH technique was found to have inherent super-resolving properties. This system has been developed to the stage of a bench-top hardware and software prototype. In this proposal, the network of 4 investigators at three universities including Vladimir Lupashin at University of Arkansas for Medical Sciences will participate in three aspects of the implementation, research use and further improvement ofthe FINCH super-resolution microscope technology. 1. At Johns Hopkins University (JHU), Brooker and Rosen will implement a stand-alone FINCH microscope, supporting algorithms and image acquisition and hologram reconstruction software to be installed at UAMS during the middle of grant Year 2. 2. At University of Arkansas for Medical Sciences (UAMS), Storrie and Lupashin will utilize the FINCH microscope to advance innovative research in membrane trafficking and Golgi organization that would not otherwise be possible. In that work, they will also compare FINCH microscope performance with other super-resolution techniques. 3. At Ben Gurion University (BGU), Rosen will continue to develop advanced algorithms and concepts based upon FINCH to improve performance and resolving power.

这项应用的三名研究人员(阿肯色州医学科学大学的斯托里、约翰霍普金斯大学和本古里安大学的布鲁克和罗森)在过去7年里一直在合作，致力于将全息术应用于荧光显微镜可以简化3D显微镜，并利用全息术提供的超分辨率能力。这项工作得到了NSF、NIH SBIR和NIST拨款的支持，现在已经产生了一种具有超分辨特性的三维荧光显微技术。它基于一种名为Finch的新全息概念，该概念由两名研究人员(罗森和布鲁克)开发。Finch是一种全息技术，其中非相干荧光显微镜发射通过简单的设置成像，该设置由插入到标准荧光显微镜支架的光路中的空间光调制器、附加的数码相机和非激光的外部快门光源组成。最近，Finch技术被发现具有固有的超分辨特性。该系统已发展到台式硬件和软件样机阶段。在这项提案中，包括弗拉基米尔·卢帕申在内的三所大学的4名调查人员组成的网络阿肯色州医科大学将从实施、研究使用和进一步改进芬奇超分辨率显微镜技术三个方面进行研究。 1.在约翰霍普金斯大学(JHU)，Brooker和Rosen将安装一个独立的Finch显微镜，支持算法以及图像采集和全息重建软件，将于第二年赠款中期安装在UAMS。 2.在阿肯色医学大学(UAMS)，Storrie和Lupashin将利用Finch显微镜推进膜贩运和高尔基组织的创新研究，否则这是不可能的。在这项工作中，他们还将比较芬奇显微镜和其他超分辨率技术的性能。 3.在本古里安大学(BGU)，罗森将继续开发基于Finch的先进算法和概念，以提高性能和分辨率。