Development of a Motionless 3D Fluorescent Microscope

静止 3D 荧光显微镜的开发

• 批准号: 8648830
• 负责人: Gary Brooker
• 金额: $ 81.45万
• 依托单位: CELLOPTIC, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8648830
• 关键词: Complement; Complex; Computer software; Computers; Country; Development; Devices; Diagnostic; Disease; Dyes; Fluorescence Microscopy; Fluorescent Dyes; Hand; Health Professional; Holography; Image; Imaging technology; International; Laboratories; Light; Lighting; Marketing; Methods; Metric; Microscope; Microscopic; Microscopy; Optics; Performance; Phase; Positioning Attribute; Process; Production; Quantum Dots; Research; Research Personnel; Resolution; Scientist; Specimen; Staging; Surgical Diagnostic Techniques; System; Techniques; Technology; Testing; Time; Work; acronyms; base; cost; cost effective; design; digital; experience; flexibility; fluorescence microscope; health care delivery; image reconstruction; improved; instrument; new product development; novel; operation; prototype; public health relevance; reconstruction; research and development; user-friendly

Project Summary/Abstract FINCH is a new imaging technology developed by Celloptic, Inc. FINCH is the acronym for Fresnel Incoherent Correlation Holography, a single beam laserless and motionless holographic principle in which a fluorescent holographic representation of the complete 3D volume is captured on a digital camera. The method obviates the need for serial stacks and captures the complete information of a 3D volume into a single hologram. Digital reconstruction of the hologram in a computer yields in focus images at any plane in a specimen. A user friendly FINCHSCOPE, which is the hardware and software implementation of the FINCH technology to microscopy will be developed during the course of this project. It will be based upon previous work done by the investigators who have been perfecting the technique during the last 6 years. In this work, several pre-production prototypes of the hardware and software will be developed and field tested under research investigator use. One version of the system will be developed as an attachment to existing fluorescent microscopes in which the FINCHSCOPE hardware is simply substituted for the camera and binocular viewing port. An import feature of the FINCHSCOPE is its ability to image microscopic objects in 3D at resolution which exceeds the optical limits in microscopy ("super-resolution"). Because of the simplicity of the FINCHSCOPE super-resolution implementation, this product will be dramatically less expensive and simpler than other super-resolution systems and thus make higher resolution fluorescent microscopy available to many more laboratories. The widespread ability of scientists and diagnostic laboratories to view specimens at improved resolution is anticipated to enhance the discovery of disease processes both in research and in diagnostics for improved delivery of health care.

项目总结/摘要 FINCH是由Celloptic，Inc.开发的一种新的成像技术。FINCH是Fresnel的缩写 非相干相关全息术是一种单光束无激光静止全息原理， 其中完整3D体积的荧光全息表示被捕获在数字照相机上， 相机该方法避免了对串行堆栈的需要，并捕获了3D图像的完整信息。 一张全息图全息图在计算机中的数字再现产生聚焦 在样品的任何平面上成像。 一个用户友好的FINCHSCOPE，它是FINCH的硬件和软件实现 将在该项目期间开发显微镜技术。它将基于 在过去的6年里，研究人员一直在完善这项技术。 在这项工作中，将开发并现场使用几个硬件和软件的预生产原型 在研究者使用下进行测试。该系统的一个版本将作为附件开发， 现有的荧光显微镜，其中FINCHSCOPE硬件简单地代替了 摄像头和双目观察口。 FINCHSCOPE的一个重要功能是它能够以3D分辨率对微观物体进行成像 这超过了显微镜的光学极限（“超分辨率”）。由于 FINCHSCOPE超分辨率的实现，这款产品将大大降低成本， 比其他超分辨率系统更简单，因此可以实现更高分辨率的荧光显微镜 提供给更多的实验室。科学家和诊断实验室的广泛能力， 以更高的分辨率观察标本，预计将有助于发现疾病过程， 在研究和诊断方面，以改善医疗保健的提供。