NOVEL TISSUE ENGINEERING MATRICES WITH CONTROLLED MICROS

具有受控微生物的新型组织工程基质

• 批准号: 2796493
• 负责人: KRISTI S. ANSETH
• 金额: $ 16.03万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2796493
• 关键词: animal tissue; biomaterial compatibility; biomaterial development /preparation; biomaterial evaluation; cartilage transplantation; cell proliferation; chondrocytes; collagen; craniofacial; extracellular matrix; gel; hydropathy; photochemistry; polymers; polyvinyls; tissue /cell culture; tissue engineering; tissue support frame

DESCRIPTION (Adapted from the applicant's abstract) The overall objective of this proposal is to develop novel polymer networks for tissue engineering that elicit desired physicochemical properties for the repair or replacement of craniofacial and orthopaedic cartilage. In particular, this proposal focuses on the development stage of intelligent matrices with controlled architectures and gelling techniques that allow imprinting of the cellular structure in the final network microstructure. The global hypothesis to be tested is that matrix materials will provide superior performance in tissue engineering cartilage when they are engineered to: 1) match the physical and mechanical properties of native cartilage; 2) incorporate cellular structural information in the hydrogel pores; and 3) allow easy structural and chemical modification through photochemistry. Specifically, the aims of the research are as follows. Specific Aim 1: to develop hydrogel matrices based on poly (vinyl alcohol) (PVA) which have controlled molecular architecture that allows intelligent engineering of properties such as mesh size, mechanical behavior, hydrophilicity, and degradability. Specific Aim 2: to modify further the aforementioned PVA hydrogels with a novel photografting technique which facilitates initiator free gelation and grafting of hydrophilic monomers to improve the immunoprotective properties and mechanical stabilization. Specific Aim 3 to evaluate these materials for their cellular compatibility (e.g., chondrocyte proliferation and extracellular matrix formation) and their performance in the correction of a cartilage defect to restore function (e.g., mechanical and eventual integrated tissue formation).

描述 （摘自申请人摘要）本研究的总体目标 建议是开发用于组织工程的新型聚合物网络， 获得所需的物理化学性质，用于修复或替换 颅面和矫形软骨。 特别是，这项建议 重点是智能矩阵的发展阶段， 结构和胶凝技术，允许印迹的细胞 最终的网络结构。 全球假设是 测试的是基质材料将在组织中提供上级性能 工程软骨，当他们被工程化：1）匹配的物理 和天然软骨的机械性能; 2）结合细胞 水凝胶孔中的结构信息;和3）允许容易的结构化 以及通过光化学进行化学修饰。 具体而言，研究的目的如下。 具体目标1： 开发基于聚乙烯醇（PVA）的水凝胶基质，其具有 可控的分子结构， 性能，例如网孔尺寸、机械性能、亲水性，以及 降解性 具体目标2：进一步改性上述PVA 具有新型光接枝技术的水凝胶 自由凝胶化和亲水性单体的接枝，以改善 免疫保护特性和机械稳定性。 具体目标3至 评价这些材料的细胞相容性（例如，软骨细胞 增殖和细胞外基质形成）和它们在 软骨缺陷的矫正以恢复功能（例如，机械 和最终的整合组织形成）。