Clickable Microgel Scaffolds for MSC Expansion and Delivery

用于 MSC 扩展和交付的可点击微凝胶支架

基本信息

  • 批准号:
    10584600
  • 负责人:
  • 金额:
    $ 53.4万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2019
  • 资助国家:
    美国
  • 起止时间:
    2019-03-05 至 2025-02-28
  • 项目状态:
    未结题

项目摘要

PROJECT SUMMARY Repair of craniofacial bone defects is an important clinical problem with significant socioeconomic impact. Bone that is traumatically injured or diseased often requires surgical repair, but 5-10% of bone fractures fail to heal and failure rates can be even higher when the patient's bone quality is compromised (e.g., osteoporotic). In these cases, stem cell-based therapies have received increasing attention as a method to improve the healing of complex craniofacial defects. The proposed research focuses on mesenchymal stem cell (MSC) therapies because of their extensive use in clinical trials, as well as the major role that MSCs play in musculoskeletal tissue homeostasis and the pathophysiology of osteoporosis. However, in vitro expansion of MSCs to therapeutically relevant numbers reduces their regenerative capacity, and afterwards, direct injection of MSCs alone often leads to low survival. The proposed research addresses this important clinical problem through an innovative materials-based strategy, namely the synthesis and assembly of tunable microgel scaffolds for MSC expansion and delivery. Using efficient “click” chemistries and by developing photoresponsive materials, we hypothesize that scaffolds can be tuned to: i) prolong the self-renewing and regenerative capacity of MSCs during in vitro expansion and ii) promote the survival and regenerative functions of delivered MSCs that will improve healing of both healthy and osteoporotic bone. Specifically, we propose to: Aim 1. Develop a hydrogel culture system for MSC expansion and quantify the effects of mechanical cues and passaging history on MSC proliferation, multipotency, secretory properties, and epigenetic landscape; Aim 2. Process the hydrogel materials into modular microgel units for MSC delivery and tailor their properties to promote MSC survival, retention and regenerative potential; and Aim 3. Test the influence of MSC expansion conditions and modular microgel delivery systems on MSC survival and bone regeneration in vivo. If successful, this project will have an important impact on public health by providing a powerful new platform for the expansion and site specific delivery of MSCs. Given the versatility of the approach, which can be applied to numerous cell delivery systems, the results will have broader implications that can extend beyond bone regeneration.
项目总结

项目成果

期刊论文数量(69)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Photo-click living strategy for controlled, reversible exchange of biochemical ligands.
Covalently tethered transforming growth factor beta in PEG hydrogels promotes chondrogenic differentiation of encapsulated human mesenchymal stem cells.
  • DOI:
    10.1007/s13346-012-0090-2
  • 发表时间:
    2012-10
  • 期刊:
  • 影响因子:
    5.4
  • 作者:
    McCall, Joshua D.;Luoma, Jacob E.;Anseth, Kristi S.
  • 通讯作者:
    Anseth, Kristi S.
Hydrogels with Reversible Mechanics to Probe Dynamic Cell Microenvironments.
Extracellular matrix protein adsorption to phosphate-functionalized gels from serum promotes osteogenic differentiation of human mesenchymal stem cells.
  • DOI:
    10.1016/j.actbio.2012.09.007
  • 发表时间:
    2013-01
  • 期刊:
  • 影响因子:
    9.7
  • 作者:
    Gandavarapu, Navakanth R.;Mariner, Peter D.;Schwartz, Michael P.;Anseth, Kristi S.
  • 通讯作者:
    Anseth, Kristi S.
Photodegradable, Photoadaptable Hydrogels via Radical-Mediated Disulfide Fragmentation Reaction.
  • DOI:
    10.1021/ma200202w
  • 发表时间:
    2011-04-26
  • 期刊:
  • 影响因子:
    5.5
  • 作者:
    Fairbanks BD;Singh SP;Bowman CN;Anseth KS
  • 通讯作者:
    Anseth KS
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

KRISTI S. ANSETH其他文献

KRISTI S. ANSETH的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('KRISTI S. ANSETH', 18)}}的其他基金

Clickable Microgel Scaffolds for MSC Expansion and Delivery
用于 MSC 扩展和交付的可点击微凝胶支架
  • 批准号:
    9884753
  • 财政年份:
    2019
  • 资助金额:
    $ 53.4万
  • 项目类别:
Photoresponsive materials to study matricellular signaling dynamics during crypt formation and fission
用于研究隐窝形成和裂变过程中基质细胞信号动力学的光响应材料
  • 批准号:
    10737202
  • 财政年份:
    2019
  • 资助金额:
    $ 53.4万
  • 项目类别:
Clickable Microgel Scaffolds for MSC Expansion and Delivery
用于 MSC 扩展和交付的可点击微凝胶支架
  • 批准号:
    10356090
  • 财政年份:
    2019
  • 资助金额:
    $ 53.4万
  • 项目类别:
Synthetic hydrogels to study formation and maintenance of intestinal crypts
用于研究肠隐窝的形成和维持的合成水凝胶
  • 批准号:
    10418728
  • 财政年份:
    2019
  • 资助金额:
    $ 53.4万
  • 项目类别:
Synthetic hydrogels to study formation and maintenance of intestinal crypts
用于研究肠隐窝的形成和维持的合成水凝胶
  • 批准号:
    9981736
  • 财政年份:
    2019
  • 资助金额:
    $ 53.4万
  • 项目类别:
Synthetic hydrogels to study formation and maintenance of intestinal crypts
用于研究肠隐窝的形成和维持的合成水凝胶
  • 批准号:
    10164770
  • 财政年份:
    2019
  • 资助金额:
    $ 53.4万
  • 项目类别:
Hydrogels to Study Synergistic Effects of Signaling Factors and Matrix Mechanics on Valve Disease Progression
水凝胶研究信号因子和基质力学对瓣膜疾病进展的协同作用
  • 批准号:
    9247569
  • 财政年份:
    2016
  • 资助金额:
    $ 53.4万
  • 项目类别:
Hydrogels to Study Synergistic Effects of Signaling Factors and Matrix Mechanics on Valve Disease Progression
水凝胶研究信号因子和基质力学对瓣膜疾病进展的协同作用
  • 批准号:
    9397567
  • 财政年份:
    2016
  • 资助金额:
    $ 53.4万
  • 项目类别:
Protease Activity in 3D Matrices
3D 矩阵中的蛋白酶活性
  • 批准号:
    8684387
  • 财政年份:
    2014
  • 资助金额:
    $ 53.4万
  • 项目类别:
Engineering Tissue with miRNAs
用 miRNA 工程组织
  • 批准号:
    8097538
  • 财政年份:
    2010
  • 资助金额:
    $ 53.4万
  • 项目类别:

相似国自然基金

多模态超声VisTran-Attention网络评估早期子宫颈癌保留生育功能手术可行性
  • 批准号:
  • 批准年份:
    2022
  • 资助金额:
    30 万元
  • 项目类别:
    青年科学基金项目
Ultrasomics-Attention孪生网络早期精准评估肝内胆管癌免疫治疗的研究
  • 批准号:
  • 批准年份:
    2022
  • 资助金额:
    52 万元
  • 项目类别:
    面上项目

相似海外基金

Development of social attention indicators of emerging technologies and science policies with network analysis and text mining
利用网络分析和文本挖掘开发新兴技术和科学政策的社会关注指标
  • 批准号:
    24K16438
  • 财政年份:
    2024
  • 资助金额:
    $ 53.4万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Improving Flexible Attention to Numerical and Spatial Magnitudes in Young Children
提高幼儿对数字和空间大小的灵活注意力
  • 批准号:
    2410889
  • 财政年份:
    2024
  • 资助金额:
    $ 53.4万
  • 项目类别:
    Continuing Grant
The Information-Attention Tradeoff: Toward an Understanding of the Fundamentals of Online Attention
信息与注意力的权衡:了解在线注意力的基本原理
  • 批准号:
    2343858
  • 财政年份:
    2024
  • 资助金额:
    $ 53.4万
  • 项目类别:
    Continuing Grant
The everyday learning opportunities of young children with attention and motor difficulties: From understanding constraints to reshaping intervention
注意力和运动困难幼儿的日常学习机会:从理解限制到重塑干预
  • 批准号:
    MR/X032922/1
  • 财政年份:
    2024
  • 资助金额:
    $ 53.4万
  • 项目类别:
    Fellowship
Towards a cognitive process model of how attention and choice interact
建立注意力和选择如何相互作用的认知过程模型
  • 批准号:
    DP240102605
  • 财政年份:
    2024
  • 资助金额:
    $ 53.4万
  • 项目类别:
    Discovery Projects
DDRIG in DRMS: Communicating risks in a sensational media environment-Using short video multimodal features to attract attention and reduce psychological reactance for persuasion
DRMS中的DDRIG:耸人听闻的媒体环境中沟通风险——利用短视频多模态特征吸引注意力,减少说服心理抵触
  • 批准号:
    2343506
  • 财政年份:
    2024
  • 资助金额:
    $ 53.4万
  • 项目类别:
    Standard Grant
Assessing the Influence of Reading Fiction on Multiple Tests of Attention
评估阅读小说对注意力多重测试的影响
  • 批准号:
    24K16033
  • 财政年份:
    2024
  • 资助金额:
    $ 53.4万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
CAREER: Designing Ultra-Energy-Efficient Intelligent Hardware with On-Chip Learning, Attention, and Inference
职业:设计具有片上学习、注意力和推理功能的超节能智能硬件
  • 批准号:
    2336012
  • 财政年份:
    2023
  • 资助金额:
    $ 53.4万
  • 项目类别:
    Continuing Grant
CPS: Small: Brain-Inspired Memorization and Attention for Intelligent Sensing
CPS:小:智能传感的受大脑启发的记忆和注意力
  • 批准号:
    2312517
  • 财政年份:
    2023
  • 资助金额:
    $ 53.4万
  • 项目类别:
    Standard Grant
CAREER: Understanding the Relationship of Covert and Overt Attention Using Concurrent EEG and Eye Tracking
职业:使用并发脑电图和眼动追踪了解隐性注意力和显性注意力的关系
  • 批准号:
    2345898
  • 财政年份:
    2023
  • 资助金额:
    $ 53.4万
  • 项目类别:
    Continuing Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了