Hydrogels to Study Synergistic Effects of Signaling Factors and Matrix Mechanics on Valve Disease Progression
水凝胶研究信号因子和基质力学对瓣膜疾病进展的协同作用
基本信息
- 批准号:9247569
- 负责人:
- 金额:$ 35.01万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2016
- 资助国家:美国
- 起止时间:2016-12-15 至 2020-11-30
- 项目状态:已结题
- 来源:
- 关键词:AddressAftercareAortic Valve StenosisAttentionBackBiochemicalCell Culture TechniquesCellsCuesCytokine SignalingDiseaseDisease ProgressionEncapsulatedEpigenetic ProcessExcisionExperimental DesignsExposure toFibroblastsFibrosisGelGoalsGrowth FactorHeart Valve DiseasesHeart ValvesHistologicHomeostasisHydrogelsIL6 geneIn SituIn VitroInflammationInflammatoryInterleukin-1 betaKnowledgeLightMechanicsMediatingMedicineMethodsMolecularMolecular TargetMorphologyMyofibroblastOperative Surgical ProceduresOrganPI3K/AKTPathogenicityPathologic ProcessesPathway AnalysisPathway interactionsPhenotypePhosphorylationPlayPopulationProcessRoleSignal PathwaySignal TransductionSignaling ProteinStenosisStimulusSystemTNF geneTestingTherapeuticTimeTissuesTransforming Growth Factor betaWorkWound Healingaortic valveaortic valve replacementclinically relevantcombinatorialculture platescytokineefficacy testingethylene glycolinhibitor/antagonistinnovationinsightinterestinterstitial cellmechanical propertiesmechanotransductionmolecular drug targetmolecular targeted therapiesmouse modelreceptorsmall molecule inhibitorstemtargeted treatmenttranscriptometranscriptome sequencingvalvular stenosis
项目摘要
Myofibroblast activation of Valvular Interstitial Cells (VICs) is considered to be a primary driver of
valvular fibrosis and stenosis. For this reason, the external cues that act to control the
myofibroblast phenotype of VICs have been topics of considerable attention in the field.
Increasing evidence suggests that beyond receptor-mediated activation of VICs by soluble growth
factors, physical cues from the matrix play a critical role in this process. Unfortunately, traditional
methods used to culture VICs inherently leads to their myofibroblast activation, such that it
becomes difficult to determine the effects of environmental stiffness on activation and especially
de-activation. To address this issue, our group has demonstrated that unique hydrogel materials
can be used to create soft, non-activating substrates for VIC culture that allow VICs to maintain a
phenotype that more closely resembles that of freshly isolated cells. Now, we aim to examine how
matrix stiffness in combination with pro-inflammatory cytokines influence the VIC fibroblast-to-
myofibroblast transition, the epigenetic changes that may occur to these cells over time, and the
pathways in matrix signaling that might be useful in reversing the pathogenic myofibroblast
phenotype. Specifically, we propose to: 1) Use a combinatorial approach to study the effect of
pro-inflammatory cytokines on VIC phenotypes as a function of microenvironmental stiffness 2)
Identify the effects of mechanical and inflammatory cues on the fibroblast-to-myofibroblast
transition and its reversal using hydrogels with dynamically tunable mechanical properties, and 3)
Discover new molecular targets for therapeutics to temper pathogenic VIC myofibroblast
activation under inflammatory conditions. Together, work completed within each of these Aims
will provide unique insight into the progression of fibrotic aortic valvular stenosis. The creation of
tunable cell culture platforms will allow us to answer questions about differences between
reversible (transient, wound healing state) and irreversible (persistent, pathogenic state) VIC
myofibroblasts that cannot be adequately addressed with traditional methods. Subsequent
analysis of the signaling pathways and genes will be used to identify new targets with therapeutic
potential to reverse VIC activation and treat valve disease. Moreover, successful completion of
these Aims should be of general interest to the field of medicine, as mechanisms of fibrosis are
likely shared among most fibrosis-related diseases.
瓣膜间质细胞(VIC)的肌成纤维细胞活化被认为是心脏病的主要驱动因素。
瓣膜纤维化和狭窄。出于这个原因,外部线索,以控制
VIC的肌成纤维细胞表型已经成为本领域相当关注的主题。
越来越多的证据表明,除了受体介导的可溶性生长激活VIC外,
在这一过程中,来自母体的物理线索起着关键作用。不幸的是,传统
用于培养VIC的方法固有地导致它们的肌成纤维细胞活化,
变得难以确定环境刚度对激活的影响,
去激活为了解决这个问题,我们的团队已经证明,独特的水凝胶材料
可用于制造用于维克培养的柔软的非活化基质,
表型更接近于新鲜分离的细胞。现在,我们的目标是研究如何
基质硬度与促炎细胞因子联合影响维克成纤维细胞对
肌成纤维细胞转变,这些细胞随时间推移可能发生的表观遗传变化,以及
可能有助于逆转致病性肌成纤维细胞
表型具体而言,我们建议:1)使用组合方法来研究
促炎细胞因子对维克表型的影响,作为微环境刚度的函数2)
确定机械和炎症因素对成纤维细胞到肌成纤维细胞的影响
使用具有动态可调机械性能的水凝胶的转变及其逆转,以及3)
发现新的分子靶点用于治疗以缓和致病性维克肌成纤维细胞
在炎症条件下活化。在这些目标范围内完成的工作
将为纤维化主动脉瓣狭窄的进展提供独特的见解。建立
可调的细胞培养平台将使我们能够回答关于
可逆(短暂、伤口愈合状态)和不可逆(持续、致病状态)维克
肌成纤维细胞不能用传统方法充分解决。后续
对信号通路和基因的分析将用于确定治疗的新靶点。
逆转维克激活和治疗瓣膜疾病的潜力。此外,圆满完成
这些目的应该是医学领域普遍感兴趣的,因为纤维化的机制是
可能是大多数纤维化相关疾病的共同特征。
项目成果
期刊论文数量(0)
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KRISTI S. ANSETH其他文献
KRISTI S. ANSETH的其他文献
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{{ truncateString('KRISTI S. ANSETH', 18)}}的其他基金
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9884753 - 财政年份:2019
- 资助金额:
$ 35.01万 - 项目类别:
Photoresponsive materials to study matricellular signaling dynamics during crypt formation and fission
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10737202 - 财政年份:2019
- 资助金额:
$ 35.01万 - 项目类别:
Clickable Microgel Scaffolds for MSC Expansion and Delivery
用于 MSC 扩展和交付的可点击微凝胶支架
- 批准号:
10356090 - 财政年份:2019
- 资助金额:
$ 35.01万 - 项目类别:
Synthetic hydrogels to study formation and maintenance of intestinal crypts
用于研究肠隐窝的形成和维持的合成水凝胶
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10418728 - 财政年份:2019
- 资助金额:
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Synthetic hydrogels to study formation and maintenance of intestinal crypts
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- 批准号:
9981736 - 财政年份:2019
- 资助金额:
$ 35.01万 - 项目类别:
Clickable Microgel Scaffolds for MSC Expansion and Delivery
用于 MSC 扩展和交付的可点击微凝胶支架
- 批准号:
10584600 - 财政年份:2019
- 资助金额:
$ 35.01万 - 项目类别:
Synthetic hydrogels to study formation and maintenance of intestinal crypts
用于研究肠隐窝的形成和维持的合成水凝胶
- 批准号:
10164770 - 财政年份:2019
- 资助金额:
$ 35.01万 - 项目类别:
Hydrogels to Study Synergistic Effects of Signaling Factors and Matrix Mechanics on Valve Disease Progression
水凝胶研究信号因子和基质力学对瓣膜疾病进展的协同作用
- 批准号:
9397567 - 财政年份:2016
- 资助金额:
$ 35.01万 - 项目类别:
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