MECHANISM OF ACTION OF GROWTH HORMONE

生长激素的作用机制

• 批准号: 2749421
• 负责人: H M GOODMAN
• 金额: $ 27.59万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-05-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2749421
• 关键词: JAK kinase; adipocytes; calcium channel; calcium flux; hormone receptor; hormone regulation /control mechanism; protein isoforms; protein structure function; receptor binding; somatotropin; tissue /cell culture

DESCRIPTION (Adapted from the applicant's abstract): In the rodent there are two isoforms of the GH receptor derived from the same gene. The long form GHRL spans the cell membrane and can signal most if not all of the effects of GH probably through activation of the cytosolic protein tyrosine kinase, JAK2. The short isoform of the receptor, GHRs consists only of the extracellular domain and a hydrophilic tail, but is somehow tethered to the cell surface where it participates in hormone binding and probably signaling. Dr. Goodman will examine the effects of the influx of ca2+ brought on by GH on the expression of the above effects and in particular the refractory response. The role of Ca2+ and its potential second messenger function as a modulator or director of the signaling cascades initiated by tyrosine phosphorylation of cellular proteins will be explored.

描述（改编自申请人的摘要）：在啮齿动物中 是源自同一基因的 GH 受体的两种亚型。 长的 GHRL 形式跨越细胞膜，可以发出大多数（如果不是全部）信号 GH 的作用可能是通过激活胞浆蛋白酪氨酸来实现的 激酶，JAK2。 受体的短亚型 GHR 仅由 细胞外结构域和亲水尾部，但以某种方式束缚在 细胞表面参与激素结合，可能 发信号。 Goodman 博士将检查 ca2+ 流入的影响 GH对上述效果的表达，特别是 难治反应。 Ca2+的作用及其潜在的第二 信使作为信号级联的调节器或指挥器 将探索由细胞蛋白质的酪氨酸磷酸化引发的过程。