CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLS

描述：根据初步结果，P.I.提出了一项 有趣而合理的中心假设，即CAPS作用于膜 通过与PIP2相互作用促进膜相互作用或融合。 私家侦探建议通过解决四个具体问题来测试这一假设。 目标。具体目标1的目标是描述CAPS的机制 行动上具体目标2的目标是开始分析 CAPS和研究CAPS在结合至 PIP2。具体目标3的目标是表征CAPS空值 神经细胞中的表型。具体目标4的目标是， 一种可能参与触发融合的新的胞质因子。

DESCRIPTION: Based on preliminary results the P.I. has proposed an interesting and reasonable central hypothesis, that CAPS acts on membranes to facilitate membrane interactions or fusion by interacting with PIP2. The P.I. proposes to test this hypothesis by addressing four specific aims. The goal of specific aim 1 is to characterize the mechanism of CAPS action. The goal of specific aim 2 is to begin to analyze the structure of CAPS and to investigate conformational changes in CAPS upon binding to PIP2. The goal of specific aim 3 is to characterize the CAPS null phenotype in neural cells. The goal of specific aim 4 is to characterize a new cytosolic factor that may be involved in triggering fusion.