CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLS

神经内分泌细胞钙分泌的调节

• 批准号: 2900215
• 负责人: THOMAS F. J. MARTIN
• 金额: $ 20.57万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-10 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2900215
• 关键词: PC12 cells; binding proteins; calcium ion; cell membrane; intermolecular interaction; membrane fusion; microinjections; molecular cloning; neuroendocrine system; phosphatidylinositols; protein structure function; restriction mapping; secretion; site directed mutagenesis; western blottings

DESCRIPTION: Based on preliminary results the P.I. has proposed an interesting and reasonable central hypothesis, that CAPS acts on membranes to facilitate membrane interactions or fusion by interacting with PIP2. The P.I. proposes to test this hypothesis by addressing four specific aims. The goal of specific aim 1 is to characterize the mechanism of CAPS action. The goal of specific aim 2 is to begin to analyze the structure of CAPS and to investigate conformational changes in CAPS upon binding to PIP2. The goal of specific aim 3 is to characterize the CAPS null phenotype in neural cells. The goal of specific aim 4 is to characterize a new cytosolic factor that may be involved in triggering fusion.

描述：根据初步结果，P.I.提出了一项 有趣而合理的中心假说，帽作用于膜 通过与PIP2相互作用促进膜相互作用或融合。 P.I.建议通过解决四个具体的问题来检验这一假设 目标。具体目标1的目标是描述CAPS的机制 行动。具体目标2的目标是开始分析 并研究CAPS结合时的构象变化 PIP2。具体目标3的目标是将大写为空 神经细胞的表型。具体目标4的目标是描述 一种新的胞浆因子，可能参与触发融合。