TERMINATION OF TRANSCIRPTION BY RNA POLYMERASE I

RNA 聚合酶 I 终止转录

• 批准号: 2608893
• 负责人: RONALD H REEDER
• 金额: $ 31.85万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-12-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2608893
• 关键词: DNA binding protein; DNA directed RNA polymerase; Saccharomyces cerevisiae; crystallization; genetic promoter element; laboratory rabbit; molecular cloning; molecular genetics; nucleic acid sequence; polymerase chain reaction; posttranscriptional RNA processing; protein structure; transcription factor; transcription termination

We will continue to explore the mechanisms involved in transcription termination by RNA polymerase l in the yeast, Saccharomyces cerevisiae. At present we know the minimal DNA sequences needed for efficient termination in vitro, we know that a protein, Reblp, binds to this sequence and acts as a terminator protein, and we have evidence that an RNA 3' processing activity associates with termination. We have proposed a model for termination that incorporates all of these elements and we will test this model in as many ways as possible. For future studies we have developed an in vivo color assay in which yeast colonies change from white to red when polI termination is active. Using this assay we will more rigorously define both DNA sequences and proteins required for termination in the living cell. This will involve doing genetic screens for proteins involved in termination as well as further defining the regions of known proteins (such as polI itself and Reblp) which are essential for termination. The RNA 3' end processing activity associated with poll is similar in action but physically distinct from the polII elongation factor, SII (TFIIS). We will purify the polI associated processing factor, clone its gene, and determine its role, if any, in the termination process. An essential part of the poll termination model is the proposition that Reblp bound to its DNA site acts as a pausing signal that is specific for poll. To determine how this occurs we intend to determine the crystal structure of the Reblp DNA binding domain co-crystallized with its cognate DNA site. Differential termination and/or blocks to elongation are used as control mechanisms for numerous human oncogenes as well as for HIV-1. At present poll termination is probably the best understood and most accessible termination system for eukaryotic RNA polymerases. We believe that insights gained in this model system will likely aid in understanding termination by other eukaryotic RNA polymerases as well.

我们将继续探索转录所涉及的机制

项目成果

The mechanism of transcription termination by RNA polymerase I.

RNA聚合酶I转录终止的机制。

• DOI: 10.1111/j.1365-2958.1994.tb00989.x
• 发表时间: 1994
• 期刊: Molecular microbiology
• 影响因子: 3.6
• 作者: Reeder,RH;Lang,W
• 通讯作者: Lang,W

Saccharomyces cerevisiae RNA polymerase I terminates transcription at the Reb1 terminator in vivo.

酿酒酵母 RNA 聚合酶 I 在体内终止 Reb1 终止子处的转录。

• DOI: 10.1128/mcb.19.11.7369
• 发表时间: 1999
• 期刊: Molecular and cellular biology
• 影响因子: 5.3
• 作者: Reeder,RH;Guevara,P;Roan,JG
• 通讯作者: Roan,JG

The yeast transcription terminator for RNA polymerase I is designed to prevent polymerase slippage.

RNA 聚合酶 I 的酵母转录终止子旨在防止聚合酶滑动。

• DOI: 10.1074/jbc.271.27.16104
• 发表时间: 1996
• 期刊: The Journal of biological chemistry
• 作者: Jeong,SW;Lang,WH;Reeder,RH
• 通讯作者: Reeder,RH

The release element of the yeast polymerase I transcription terminator can function independently of Reb1p.

酵母聚合酶 I 转录终止子的释放元件可以独立于 Reb1p 发挥作用。

• DOI: 10.1128/mcb.15.11.5929
• 发表时间: 1995
• 期刊: Molecular and cellular biology
• 影响因子: 5.3
• 作者: Jeong,SW;Lang,WH;Reeder,RH
• 通讯作者: Reeder,RH