TERMINATION OF TRANSCRIPTION BY RNA POLYMERASE I

RNA 聚合酶 I 终止转录

• 批准号: 3300200
• 负责人: RONALD H REEDER
• 金额: $ 21.88万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-12-01 至 1994-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3300200
• 关键词: DNA binding protein; DNA directed RNA polymerase; DNA footprinting; Xenopus; fresh water environment; gel electrophoresis; gene expression; genetic promoter element; ion exchange chromatography; molecular cloning; protein reconstitution; protein signal sequence; ribosomal DNA; tissue /cell culture; transcription termination

Termination of transcription is a control point that is frequently utilized in prokaryotes to regulate gene expression. However, the extent to which this is true in eukaryotes is not yet clear. At present, the termination system which is best understood in eukaryotes is that of RNA polymerase I, due largely to studies on the frog, Xenopus laevis, in our own laboratory and studies on the mouse in the laboratory of Ingrid Grummt. The sites of termination are known, various mutants of these sites are available, and good assay systems for termination have been developed both in vivo and vitro. Fractionation of the proteins involved in termination is underway and we have begun to propose testable models for the process. In this application we propose to continue studies of RNA polymerase I termination in Xenopus. The proteins involved in termination will be identified and purified using and in vitro termination system. The genes for these proteins will be cloned and characterized. The heart of the proposal then is to determine the molecular mechanism by which these components effect termination of transcription by RNA polymerase I.

转录终止是经常使用的控制点 在原核生物中调节基因表达。 然而，在多大程度上 这在真核生物中是否属实尚不清楚。 目前，终止 真核生物中最容易理解的系统是 RNA 聚合酶 I 的系统， 主要归功于我们自己实验室对青蛙（Xenopus laevis）的研究 以及 Ingrid Grummt 实验室对小鼠的研究。 的站点 终止是已知的，这些位点的各种突变体是可用的，并且 良好的终止检测系统已经在体内和体外开发出来。 体外。 参与终止的蛋白质的分离正在进行中 我们已经开始为这个过程提出可测试的模型。 在此应用中，我们建议继续研究 RNA 聚合酶 I 非洲爪蟾的终止。 参与终止的蛋白质将是 使用体外终止系统进行鉴定和纯化。 基因 这些蛋白质将被克隆和表征。 的心 然后的建议是确定这些物质的分子机制 成分影响 RNA 聚合酶 I 的转录终止。