ORGANIC SECONDARY ION IMAGING

有机二次离子成像

• 批准号: 2614284
• 负责人: PETER Justin TODD
• 金额: $ 17.74万
• 依托单位: LOCKHEED MARTIN ENERGY RESEARCH CORP
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2614284
• 关键词: biomedical equipment development; brain mapping; electrospray ionization mass spectrometry; laboratory rat; phospholipids; phosphorylcholine; tissue /cell preparation

DESCRIPTION: (applicant's abstract) The objective of this research is development of secondary ion probe - ion trap mass spectrometry for determining the spatial and compositional distribution of targeted organic compounds in biological tissue. The particular targets of the current research period are glcycerophospholipids distributed in the brains of adult and neonatal rats. To accomplish these analyses, a secondary ionization (SI) microprobe will be integrated with an ion trap to create a SIMS/MS microprobe. The ion trap addition will permit high sensitivity in the measurement of ions of m/z 600-1000; this enhanced sensitivity is essential to mapping glycerophospholipids. This instrument makes it possible to generate ions from a particular location on the sample using a finely focused beam of the fullerene primary ions. Use of fullerene primary ions makes it possible to continually erode the sample surface without causing chemical alterations of the sample, at least as evident in successive SIMS spectra. This feature will make it possible to develop not only images based on the distribution of a single constituent, but maps of the different distributions of related compounds can be determined. Maps based upon phosphocholine secondary ion emission from adult rat brains will be developed initially, since similar maps have been generated using triple quadrupole mass spectrometry, and the distribution of phosphatidylcholine in adult rats is fixed.

描述：(申请人摘要)本研究的目的是 二次离子探针-离子陷阱质谱仪的研制 确定目标有机污染物的空间和组成分布 生物组织中的化合物。当前的特定目标 研究期间甘油磷脂在成人脑中的分布 和新生大鼠。为了完成这些分析，二次电离 (Si)微探头将与离子陷阱集成，以创建SIMS/MS 微探头。离子陷阱的加入将允许在 测量m/z 600-1000的离子；这种增强的灵敏度是必不可少的 测绘甘油磷脂。这台仪器使我们有可能 从样本上的特定位置使用精细的 富勒烯初级离子的聚焦光束。富勒烯初级离子的使用 使其能够持续侵蚀样品表面而不会导致 样品的化学变化，至少在连续的SIMs中是明显的 光谱。此功能将使开发不仅是图像成为可能 基于单一成分的分布，但不同的地图 可以确定相关化合物的分布。基于以下条件的地图 成年大鼠脑内的磷胆碱次级离子发射将是 最初开发，因为已经使用三元组生成了类似的地图 四极杆质谱仪，以及磷脂酰胆碱在体内的分布 成年大鼠是固定的。