ORGANIC SECONDARY ION IMAGING

有机二次离子成像

• 批准号: 3299878
• 负责人: PETER Justin TODD
• 金额: $ 11.21万
• 依托单位: LOCKHEED MARTIN ENERGY SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3299878
• 关键词: clinical biomedical equipment; computer data analysis; image processing; mass spectrometry

The objective of this research is development of a method to determine spatial distributions of targeted organic compounds in biological tissue. Such a capability would make possible rapid analysis of tissue for localization of toxins and pharmaceuticals, and quite possibly, their metabolites. Secondary ion mass spectrometry (SIMS) will be employed to obtain organic ion micrographs of the tissue. The spatial resolution which can be attained will be determined by the practical yield of secondary ions, that is, the number of analyte-characteristic secondary ions detected per incident primary ion. Instrumental capabilities will be optimized by rigorous application of ion optical design criteria to the entire secondary ion mass spectrometer. Methods of in situ chemical pretreatment of the sample will be developed for enhancing characteristic secondary ion emission from selected analytes. Choice of primary ion beam species and conditions will be investigated to optimize secondary ion yield. Tandem mass spectroscopy (MS/MS) will be employed so that characteristic ions can be resolved by their structure, in addition to their mass-to-charge ratios, as is currently practiced in SIMS. Data will be obtained using an existing IBM PC/XT data system, with off-line data manipulation and image processing performed by a DEC MicroVAX computer.

本研究的目的是开发一种方法来确定 生物组织中目标有机化合物的空间分布。 这种能力将使快速分析组织成为可能 毒素和药物的定位，很可能是它们的定位 代谢物。 二次离子质谱（SIMS）将用于 获得组织的有机离子显微照片。 空间分辨率 能否达到，取决于二级的实际产量 离子，即检测到的分析物特征二次离子的数量 每个事件一次离子。 仪器功能将通过以下方式优化 离子光学设计标准严格应用于整个次级 离子质谱仪。 原位化学预处理方法 将开发样品以增强特征二次离子 选定分析物的发射。 主要离子束种类的选择和 将研究条件以优化二次离子产率。 串联 将采用质谱法 (MS/MS)，以便可以分析特征离子 除了它们的质荷比之外，还可以通过它们的结构来解决， 正如 SIMS 目前所采用的那样。 数据将使用现有的 IBM PC/XT数据系统，具有离线数据操作和图像处理功能 由 DEC MicroVAX 计算机执行。