ORGANIC SECONDARY ION IMAGING

有机二次离子成像

• 批准号: 3299879
• 负责人: PETER Justin TODD
• 金额: $ 13.14万
• 依托单位: LOCKHEED MARTIN ENERGY SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3299879
• 关键词: clinical biomedical equipment; computer data analysis; image processing; mass spectrometry

The objective of this research is development of a method to determine spatial distributions of targeted organic compounds in biological tissue. Such a capability would make possible rapid analysis of tissue for localization of toxins and pharmaceuticals, and quite possibly, their metabolites. Secondary ion mass spectrometry (SIMS) will be employed to obtain organic ion micrographs of the tissue. The spatial resolution which can be attained will be determined by the practical yield of secondary ions, that is, the number of analyte-characteristic secondary ions detected per incident primary ion. Instrumental capabilities will be optimized by rigorous application of ion optical design criteria to the entire secondary ion mass spectrometer. Methods of in situ chemical pretreatment of the sample will be developed for enhancing characteristic secondary ion emission from selected analytes. Choice of primary ion beam species and conditions will be investigated to optimize secondary ion yield. Tandem mass spectroscopy (MS/MS) will be employed so that characteristic ions can be resolved by their structure, in addition to their mass-to-charge ratios, as is currently practiced in SIMS. Data will be obtained using an existing IBM PC/XT data system, with off-line data manipulation and image processing performed by a DEC MicroVAX computer.

本研究的目的是发展一种方法来确定