TIME-RESOLVED MAGNETIC CIRCULAR DICHROISM
TIME-RESOLVED MAGNETIC CIRCULAR DICHROISM
批准号:
2684860
负责人:
DAVID S. KLIGER
金额:
$21.48万
依托单位国家:
美国
项目类别:
财政年份:
1987
资助国家:
美国
项目状态:
已结题
起止时间:
1987-07-01 至 2000-03-31
关键词:
biomedical equipment development chemical kinetics circular magnetic dichroism conformation cytochrome b cytochrome c cytochrome oxidase electron transport enzyme mechanism enzyme structure hemoglobin hemoprotein structure magnetic field method development molecular dynamics mutant photochemistry photolysis protein structure function structural biology time resolved data ultraviolet radiation
中文摘要
描述:时间分辨磁循环的开发和使用
二色性光谱(TRMCD)。 的应用
磁场诱导手性,并且观察到的信号通常是
定位于被探测吸收的部分。 这
技术用于血红素中心。将技术扩展到
近紫外线是要开发,和初步数据的静态MCD的
色氨酸是令人鼓舞的(将需要一个额外的电磁铁,
补偿单元窗口的法拉第旋转)。 中央
想法是光解配体,然后研究动力学。 的
TRMCD装置在血红素中心的分辨率约为50-100 ns
吸收(部分由蛋白质产生的信号复杂化)
扩散重定向时间,约100 ns-这种并发症已经
分析并可能纠正)。 细胞色素氧化酶的研究,
细胞色素C3和细胞色素C'正在进行中。 一个特别重要
过去一段时间的发现是在
细胞色素氧化酶的细胞色素a3位点,导致部分模型
门控机制的耦合能量释放氧还原,
质子泵(配体穿梭模型)。一种未知的配体
蛋白质与蛋白质铁中心结合,
用这种技术。 未来最有趣的研究集中在
血红蛋白和R到T变构转换,如果色氨酸残基可以
隔离和测量。静态MCD显示了一些希望
在溶液中的色氨酸。突变体应该分离出哪个trp残基产生
信号了最近的TRCD结果,刚刚提交给生物化学,
令人兴奋.杰出的研究,除了有两个问题,
上一篇:蛋白质取向弛豫问题,
在100 ns的时间尺度上,以及生产率的问题。
英文摘要
DESCRIPTION: Development and use of time resolved magnetic circular
dichroism spectroscopy (TRMCD) is proposed. The application of a
magnetic field induces chirality and the observed signal is generally
localized to the moiety who's absorption is being probed. This
technique is used for heme centers. Extension of the technique to the
near UV is to be developed, and preliminary data on the static MCD of
tryptophan is encouraging (will require an extra electromagnet to
compensate for the Faraday rotation of the cell windows). The central
idea is to photolyze a ligand and then study the kinetics. The
resolution of the TRMCD apparatus is about 50-100 ns in the heme center
absorption (partly complicated by signals that result from the protein
diffusional reorientation time, about 100 ns- this complication has been
analyzed and is probably correctable). Studies on cytochrome oxidase,
cytochrome c3, and cytochrome c' are ongoing. A particularly important
finding of the past period was the finding of a transient ligation at the
cytochrome a3 site of cytochrome oxidase, resulting in a model for part
of the gating mechanism coupling energy release by oxygen reduction to
proton pumping (the ligand shuttle model). An unknown ligand in the
protein binds to the protein iron center and this has been best studied
with this technique. Most interesting future studies center on
hemoglobin and the R to T allosteric transition, if the trp residues can
be isolated and measured. Some hope has been demonstrated by static MCD
on trp in solution. Mutants should isolate which trp residues yield
signal. Recent TRCD results, just submitted to Biochemistry, are
exciting. Outstanding studies except there were two problems cited in
last review: the problem of protein orientational relaxation, which is
on the 100 ns time scale, and the issue of productivity.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Circular Dichroism Spectropolarimeter
-
批准号:8639931
-
项目类别:
-
资助金额:$13.2万
-
财政年份:2014
-
负责人:DAVID S. KLIGER
-
依托单位:
Fast Kinetic Studies of Protein Folding and Function
-
批准号:7098013
-
项目类别:
-
资助金额:$32.38万
-
财政年份:2004
-
负责人:DAVID S. KLIGER
-
依托单位:
Fast Kinetic Studies of Protein Folding and Function
-
批准号:6874802
-
项目类别:
-
资助金额:$32.59万
-
财政年份:2004
-
负责人:DAVID S. KLIGER
-
依托单位:
Fast Kinetic Studies of Protein Folding and Function
-
批准号:7270641
-
项目类别:
-
资助金额:$31.42万
-
财政年份:2004
-
负责人:DAVID S. KLIGER
-
依托单位:
Fast Kinetic Studies of Protein Folding and Function
-
批准号:6945191
-
项目类别:
-
资助金额:$33.18万
-
财政年份:2004
-
负责人:DAVID S. KLIGER
-
依托单位:
SMALL INSTRUMENTATION GRANT
-
批准号:3524856
-
项目类别:
-
资助金额:$2.14万
-
财政年份:1991
-
负责人:DAVID S. KLIGER
-
依托单位:
TIME-RESOLVED MAGNETIC CIRCULAR DICHROISM
-
批准号:6519266
-
项目类别:
-
资助金额:$24.17万
-
财政年份:1987
-
负责人:DAVID S. KLIGER
-
依托单位:
TIME-RESOLVED MAGNETIC CIRCULAR DICHROISM
-
批准号:2900657
-
项目类别:
-
资助金额:$18.05万
-
财政年份:1987
-
负责人:DAVID S. KLIGER
-
依托单位:
TIME-RESOLVED MAGNETIC CIRCULAR DICHROISM
-
批准号:6635957
-
项目类别:
-
资助金额:$24.16万
-
财政年份:1987
-
负责人:DAVID S. KLIGER
-
依托单位:
TIME-RESOLVED MAGNETIC CIRCULAR DICHROISM
-
批准号:6127433
-
项目类别:
-
资助金额:$27.97万
-
财政年份:1987
-
负责人:DAVID S. KLIGER
-
依托单位:
TIME-RESOLVED MAGNETIC CIRCULAR DICHROISM
-
批准号:6385684
-
项目类别:
-
资助金额:$24.0万
-
财政年份:1987
-
负责人:DAVID S. KLIGER
-
依托单位:
TRANSIENT MAGNETIC CIRCULAR DICHROISM
-
批准号:3295069
-
项目类别:
-
资助金额:$15.77万
-
财政年份:1987
-
负责人:DAVID S. KLIGER
-
依托单位:
TRANSIENT MAGNETIC CIRCULAR DICHROISM
-
批准号:3295063
-
项目类别:
-
资助金额:$13.01万
-
财政年份:1987
-
负责人:DAVID S. KLIGER
-
依托单位:
TRANSIENT MAGNETIC CIRCULAR DICHROISM
-
批准号:3295064
-
项目类别:
-
资助金额:$14.12万
-
财政年份:1987
-
负责人:DAVID S. KLIGER
-
依托单位:
TIME-RESOLVED MAGNETIC CIRCULAR DICHROISM
-
批准号:2179408
-
项目类别:
-
资助金额:$19.24万
-
财政年份:1987
-
负责人:DAVID S. KLIGER
-
依托单位:
TRANSIENT MAGNETIC CIRCULAR DICHROISM
-
批准号:3295066
-
项目类别:
-
资助金额:$9.79万
-
财政年份:1987
-
负责人:DAVID S. KLIGER
-
依托单位:
TRANSIENT MAGNETIC CIRCULAR DICHROISM
-
批准号:3295065
-
项目类别:
-
资助金额:$10.53万
-
财政年份:1987
-
负责人:DAVID S. KLIGER
-
依托单位:
TIME-RESOLVED MAGNETIC CIRCULAR DICHROISM
-
批准号:2392036
-
项目类别:
-
资助金额:$19.91万
-
财政年份:1987
-
负责人:DAVID S. KLIGER
-
依托单位:
TRANSIENT MAGNETIC CIRCULAR DICHROISM
-
批准号:2179406
-
项目类别:
-
资助金额:$16.18万
-
财政年份:1987
-
负责人:DAVID S. KLIGER
-
依托单位:
TRANSIENT MAGNETIC CIRCULAR DICHROISM
-
批准号:3295067
-
项目类别:
-
资助金额:$13.7万
-
财政年份:1987
-
负责人:DAVID S. KLIGER
-
依托单位:
海外基金