TRANSIENT MAGNETIC CIRCULAR DICHROISM

瞬态磁性圆二色性

• 批准号: 3295064
• 负责人: DAVID S. KLIGER
• 金额: $ 14.12万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1995-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3295064
• 关键词: circular magnetic dichroism; cytochromes; electron transport; hemoprotein; hydrogen transport; magnetic field; metalloporphyrins; photochemistry; photolysis

This application seeks funds for continued time-resolved magnetic circular dichroism (TRMCD) studies of biological systems. The previous grant period demonstrated that it is possible to measure MCD spectra with nanosecond time resolution. It is now proposed that this technique be applied to several problems of biomedical importance. These fall into three areas: the study of intermediates which result from ligand photolysis from heme proteins and metalloporphyrins, the study of electron and proton transfer reactions in tautomerization reactions and in heme proteins, and the study of MCD spectra of molecular excited states. A few of these studies are aimed at further development of the TRMCD technique or development of theoretical frameworks needed to interpret MCD spectra. In these, artifacts which could interfere with TRMCD measurements will be investigated to be sure they are understood and can be eliminated under our experimental conditions. Also, simple systems will be studied which will be amenable to straightforward theoretical treatment so that these treatments can be optimized for understanding MCD spectral features. Most of the studies, however, will use the techniques already proven to be effective to study a variety of protein systems. These are aimed at understanding the nature of ligand, electron, or proton transfer reactions in proteins. These are critical processes which are responsible for a wide variety of processes necessary for life from transport of oxygen in the blood and muscles to production of species which can be used for energy sources by cells.

该应用程序寻求持续时间分辨的磁性圆形的资金 生物系统研究的二科主义研究。 上一个赠款期 证明可以用纳秒来测量MCD光谱 时间分辨率。 现在建议将此技术应用于 生物医学重要性的几个问题。 这些属于三个领域： 由血红素的配体光解引起的中间体的研究 蛋白质和金属丁林，电子和质子转移的研究 互变异反应和血红素蛋白中的反应以及该研究 分子激发态的MCD光谱。 其中一些研究是 旨在进一步开发TRMCD技术或开发 解释MCD光谱所需的理论框架。 在这些中， 可能会干扰TRMCD测量结果的伪像 被调查以确保它们已被理解，并且可以在我们的下被消除 实验条件。 另外，将研究简单的系统 可以直接理论处理，以便这些 可以优化治疗以了解MCD光谱特征。 最多 但是，研究将使用已经被证明是的技术 有效研究各种蛋白质系统。 这些是针对的 了解配体，电子或质子转移反应的性质 在蛋白质中。 这些是关键过程，负责广泛 生命所必需的各种过程从氧气运输中 血液和肌肉生产物种可用于能量的物种 细胞来源。