Cryoprobe for 600 MHz Biomolecular NMR

描述(由申请人提供)：申请一种高灵敏度的冷冻探头，以提高现有600 MHz核磁共振系统的灵敏度和吞吐量。该系统目前被三个主要用户广泛用于生物分子核磁共振研究：何健博士：关于血红蛋白的生物物理和分子生物学研究。Miguel Llinas博士：纤溶酶原及相关基因组结构域的核磁共振研究。戈登博士：谷胱甘肽转移酶的结构和动力学。所要求的冷冻探头也将支持佩唐博士(小使用者)在研究麻醉作用的分子基础方面所做的努力。总之，低温探头/低温平台将提高仪器的灵敏度和吞吐量。灵敏度的提高将使研究小组能够进行目前在我们现有仪器上不实用的实验。此外，灵敏度的提高将极大地增加仪器的吞吐量。效率的提高将使国家卫生研究院支持的研究项目加速实现其目标。此外，参与的调查人员还可以探索由于仪器时间和灵敏度的限制而不可能采用的其他实验系统和/或方法。最后，系统吞吐量的增加将使该设施能够容纳更多需要高场仪器的临时用户。

DESCRIPTION (provided by applicant): A request is made for a high sensitivity cryoprobe to increase the sensitivity and throughput of an existing modern 600 MHz NMR system. This system is currently heavily used for biomolecular NMR studies by three major users: Dr. Chien Ho: Biophysical and Molecular Biological Studies on Hemoglobin. Dr. Miguel Llinas: NMR Studies of Plasminogen and Related Genomic Domains. Dr. Gordon Rule: Structure and Dynamics of Glutathione transferases. The requested cryoprobe will also support the research efforts of Dr. Pei Tang (minor user) in her research efforts directed at investigating the molecular basis of anesthetic action. In summary, the cryoprobe/cryoplatform will increase the sensitivity and throughput of the instrument. The increase in sensitivity will allow the research groups to perform experiments that are currently not practical on our existing instruments. In addition, the increase in sensitivity will dramatically increase the throughput of the instrument. This increase in efficiency will allow the NIH supported research projects to progress towards their goals in an accelerated fashion. In addition, it will be possible for the participating investigators to explore additional experimental systems and/or approaches that were not possible due to the constraints imposed by limitations in instrument time and sensitivity. Finally, the increased throughput of the system will allow the facility to accommodate a larger number of occasional users who have a need for high-field instrumentation.