Cryoprobe for 600 MHz Biomolecular NMR

描述（由申请人提供）：要求提供一种高灵敏度冷冻探针，以提高现有现代600 MHz NMR系统的灵敏度和通量。该系统目前被三个主要用户大量用于生物分子NMR研究：何建：血红蛋白的生物物理和分子生物学研究。 Miguel Llinas博士：纤溶酶原和相关基因组结构域的NMR研究。Gordon Rule博士：Glucose transferases的结构和动力学。申请的冷冻探针还将支持Pei Tang博士（次要用户）的研究工作，其研究工作旨在研究麻醉作用的分子基础。总之，冷冻探针/冷冻平台将增加仪器的灵敏度和通量。灵敏度的提高将使研究小组能够在我们现有的仪器上进行目前不实用的实验。此外，灵敏度的提高将显著提高仪器的通量。这种效率的提高将使NIH支持的研究项目能够以更快的方式朝着目标前进。此外，参与的研究者将有可能探索其他实验系统和/或方法，这些系统和/或方法由于仪器时间和灵敏度的限制而无法实现。最后，系统吞吐量的增加将使该设施能够容纳更多需要高场仪器的临时用户。

DESCRIPTION (provided by applicant): A request is made for a high sensitivity cryoprobe to increase the sensitivity and throughput of an existing modern 600 MHz NMR system. This system is currently heavily used for biomolecular NMR studies by three major users: Dr. Chien Ho: Biophysical and Molecular Biological Studies on Hemoglobin. Dr. Miguel Llinas: NMR Studies of Plasminogen and Related Genomic Domains. Dr. Gordon Rule: Structure and Dynamics of Glutathione transferases. The requested cryoprobe will also support the research efforts of Dr. Pei Tang (minor user) in her research efforts directed at investigating the molecular basis of anesthetic action. In summary, the cryoprobe/cryoplatform will increase the sensitivity and throughput of the instrument. The increase in sensitivity will allow the research groups to perform experiments that are currently not practical on our existing instruments. In addition, the increase in sensitivity will dramatically increase the throughput of the instrument. This increase in efficiency will allow the NIH supported research projects to progress towards their goals in an accelerated fashion. In addition, it will be possible for the participating investigators to explore additional experimental systems and/or approaches that were not possible due to the constraints imposed by limitations in instrument time and sensitivity. Finally, the increased throughput of the system will allow the facility to accommodate a larger number of occasional users who have a need for high-field instrumentation.