NOVEL SCREEN FOR TARGETS OF THE DROSOPHILA VND GENE

果蝇 VND 基因靶标的新型筛选

• 批准号: 2463114
• 负责人: DERVLA M MELLERICK-DRESSLER
• 金额: $ 7.61万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-08 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2463114
• 关键词: DNA binding protein; Drosophilidae; developmental genetics; developmental neurobiology; fungal genetics; gel mobility shift assay; gene expression; genetic mapping; genetic transcription; in situ hybridization; molecular cloning; molecular site; neurogenesis; neurogenetics; northern blottings; nucleic acid sequence; polymerase chain reaction; southern blotting; transcription factor; western blottings

DESCRIPTION: (Adapted from applicant's description) The long term goals of this application are to elucidate the molecular mechanisms underlying neuronal specification. Toward these goals, the investigator plans to perform a mini-screen to determine whether novel and known targets of the Drosophila vnd NK-2 gene can be identified using yeast genetics to functionally screen for sequences that mediate transcriptional activation in a factor-dependent manner. If the sequences are novel, large genomic clones corresponding to vnd/NK-2 binding sites will be identified and the map position determined on polytene chromosomes. The expression pattern of both known and novel genes associated with putative vnd/NK-2 binding sites will be determined by in situ hybridization relative to vnd/NK-2 expression patterns.

描述：（根据申请人的描述改编） 本申请旨在阐明 神经元特化 为了实现这些目标，研究人员计划 进行微型筛选，以确定是否有新的和已知的目标， 果蝇vnd NK-2基因可以用酵母遗传学鉴定， 功能性筛选介导转录激活的序列， 一种依赖因素的方式。 如果序列是新的，则大型基因组克隆 将鉴定对应于vnd/NK-2结合位点的图谱， 在多线染色体上确定的位置。 两者的表达模式 与推定的vnd/NK-2结合位点相关的已知和新基因将 通过相对于vnd/NK-2表达的原位杂交测定 模式.