NOVEL SCREEN FOR TARGETS OF THE DROSOPHILA VND GENE

果蝇 VND 基因靶标的新型筛选

• 批准号: 2838837
• 负责人: DERVLA M MELLERICK-DRESSLER
• 金额: $ 7.62万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-08 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2838837
• 关键词: DNA binding protein; Drosophilidae; developmental genetics; developmental neurobiology; fungal genetics; gel mobility shift assay; gene expression; genetic mapping; genetic transcription; in situ hybridization; molecular cloning; molecular site; neurogenesis; neurogenetics; northern blottings; nucleic acid sequence; polymerase chain reaction; southern blotting; transcription factor; western blottings

DESCRIPTION: (Adapted from applicant's description) The long term goals of this application are to elucidate the molecular mechanisms underlying neuronal specification. Toward these goals, the investigator plans to perform a mini-screen to determine whether novel and known targets of the Drosophila vnd NK-2 gene can be identified using yeast genetics to functionally screen for sequences that mediate transcriptional activation in a factor-dependent manner. If the sequences are novel, large genomic clones corresponding to vnd/NK-2 binding sites will be identified and the map position determined on polytene chromosomes. The expression pattern of both known and novel genes associated with putative vnd/NK-2 binding sites will be determined by in situ hybridization relative to vnd/NK-2 expression patterns.

描述：(根据申请者描述改编)长期目标 这一应用是为了阐明潜在的分子机制。 神经元规格。为了实现这些目标，调查人员计划 执行小屏幕以确定是否新的和已知的目标 利用酵母菌遗传学鉴定果蝇vND NK-2基因 从功能上筛选介导转录激活的序列 一种依赖因素的方式。如果序列是新的、大的基因组克隆 将确定与VND/NK-2结合位点相对应的MAP 在多线染色体上确定的位置。两者的表达模式 与可能的vnd/NK-2结合位点相关的已知和新基因将 通过原位杂交检测vND/NK-2的表达 模式。