NOVEL SCREEN FOR TARGETS OF THE DROSOPHILA VND GENE

描述：(根据申请者描述改编)长期目标 这一应用是为了阐明潜在的分子机制。 神经元规格。为了实现这些目标，调查人员计划 执行小屏幕以确定是否新的和已知的目标 利用酵母菌遗传学鉴定果蝇vND NK-2基因 从功能上筛选介导转录激活的序列 一种依赖因素的方式。如果序列是新的、大的基因组克隆 将确定与VND/NK-2结合位点相对应的MAP 在多线染色体上确定的位置。两者的表达模式 与可能的vnd/NK-2结合位点相关的已知和新基因将 通过原位杂交检测vND/NK-2的表达 模式。

DESCRIPTION: (Adapted from applicant's description) The long term goals of this application are to elucidate the molecular mechanisms underlying neuronal specification. Toward these goals, the investigator plans to perform a mini-screen to determine whether novel and known targets of the Drosophila vnd NK-2 gene can be identified using yeast genetics to functionally screen for sequences that mediate transcriptional activation in a factor-dependent manner. If the sequences are novel, large genomic clones corresponding to vnd/NK-2 binding sites will be identified and the map position determined on polytene chromosomes. The expression pattern of both known and novel genes associated with putative vnd/NK-2 binding sites will be determined by in situ hybridization relative to vnd/NK-2 expression patterns.