EFFECT OF PERFUSION MEDIA ON MYOCARDIAL OXYGEN TENSION IN ISOLATED RAT HEARTS

灌注介质对离体大鼠心脏心肌氧张力的影响

• 批准号: 6123371
• 负责人: BRUCE J FRIEDMAN
• 金额: $ 1.3万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-15 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6123371
• 关键词: animal tissue; biomedical resource; cardiovascular system; gas; model design /development

In many experiments with "the isolated heart", typically it is perfused with crystalloid media equilibrated with 95% O2 to ensure adequate myocardial oxygen tension, is commonly used to study cardiac function in order to provide sufficient oxygen for metabolic demands. When hemoglobin is available for oxygen transport, however, equilibration with 21% O2 is adequate to meet metabolic demands. This study uses EPR oximetry forthe measurement of myocardial pO2 in isolated hearts perfused with an erythrocyte suspension. Baseline myocardial pO2 in erythrocyte-perfused hearts was 16.4q3.5 mmHg(meanqS.E.). When compared to previous measurements of myocardial pO2 in isolated hearts perfused with crystalloid media the use of erythrocyte suspensions resulted in a tenfold lower level of myocardial pO2 while avoiding very low and high values. The standard use of 95% oxygen with crystalloid results in myocardial levels of oxygen far above those usually found in the presence of hemoglobin and room air, while still not eliminating the potential for very low pO2 to occur in the heart muscle. Extremes of pO2 may cause physiological abnormalities and damage.

在许多“离体心脏”实验中，通常是 用 95% O2 平衡的晶体介质灌注，以确保 充足的心肌氧分压，常用于研究心脏 功能，以便为代谢需求提供足够的氧气。 然而，当血红蛋白可用于氧气运输时， 21% O2 的平衡足以满足代谢需求。 这 研究使用 EPR 血氧测定法测量心肌 pO2 用红细胞悬液灌注离体心脏。 基线 红细胞灌注心脏的心肌 pO2 为 16.4q3.5 毫米汞柱（平均qS.E.）。与之前的心肌测量结果相比 使用晶体介质灌注离体心脏中的 pO2 红细胞悬浮液导致血红蛋白水平降低十倍 心肌 pO2 同时避免非常低和高的值。 标准 使用 95% 的氧气和晶体会导致心肌水平 氧气含量远远高于血红蛋白存在时通常发现的氧气含量 室内空气，但仍无法消除氧分压极低的可能性 发生在心肌中。 极端的 pO2 可能会导致生理 异常和损坏。