ASPARAGINE LINKED OLIGOSACCHS ON CELLOBELLOHYDROLASE I FROM TRICHODERMA REESEI

里氏木霉纤维纤维素水解酶 I 上的天冬酰胺连接寡糖

• 批准号: 6123249
• 负责人: ROLAND CONTRERAS
• 金额: --
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6123249
• 关键词: biological products; biomaterials; biomedical resource; carbohydrates; immunology; infection; inflammation; lymphatic system; plants; spectrometry; structural biology

The protein glycosylation pathway still holds many questions in the case of lower eukaryotes. Thus, while the characteristics of some carbohydrate chains isolated from glycoproteins of certain filamentous fungi such as Aspergillus and Trichoderma resemble those found in yeast, others resemble those encountered in mammals. In an attempt to shed some light on the glycosylation in filamentous fungi, we have studied the predominant N-glycans from cellobiohydrolase 1, secreted from Trichoderma reesei RUTC 30. One of the compounds found is a novel phosphate-containing oligosaccharide that, to the best of our knowledge, has not been reported before. The primary structure of the novel phosphate-containing oligosaccharide, isolated from T reesei cellobiohydrolase 1, was determined by ES1 MS and found to comprise Glcl-6P03-Man7-9GlcNAC2. The Glu extensions appear to be on the lower 3-branched antenna. Thepresence of mannose residues linked through a phosphate diester resembles glycosyl synthesisin yeast. Several additional samples are under investigation.

蛋白质糖基化途径仍然存在许多问题。 低等真核生物的情况。因此，虽然一些人的特点 从某些丝状糖蛋白中分离出的碳水化合物链 曲霉和木霉等真菌与在 酵母菌，其他的类似于哺乳动物。为了试图 揭示了丝状真菌中的糖基化，我们有 研究了纤维二糖水解酶1分泌的主要N-糖链 来自里氏木霉RUTC 30。发现的一种化合物是一种 新型含磷低聚糖，尽我们所能 知识，以前没有报道过。的主要结构 一种新的含磷低聚糖，从红松中分离得到 纤维二糖水解酶1，经ES1MS测定，其组成为 GlCL-6P03-MAN7-9GlcNAC2。GLU延伸部似乎在较低的位置 三分支天线。甘露糖残基的存在通过一种 磷酸二酯类似于酵母中的糖基合成。几个 其他样本正在调查中。