AGONIST SPECIFIC ATP RELEASE FROM ENDOTHELIAL CELLS

说明： 我们对心脏EC的兴趣源于这样一个事实：心血管 疾病仍然是我们社会的一个主要健康问题。我们相信 参与心脏冠脉内皮细胞释放的因子 局部血流的调节可能在正常心脏中起作用 功能和心血管疾病。事实证明， 血管内皮细胞(EC)释放三磷酸腺苷(ATP)和 细胞外的自体刺激物对各种刺激的反应。一直以来 提示这些细胞释放的三磷酸腺苷是由受体介导的。它是 P2/y受体可能在介导ATP释放中起作用。 我们想要验证这样的假设，即心脏内皮细胞在反应中释放三磷酸腺苷 对激动剂的影响在发光和发光两种情况下都发生，并测试 缺氧和复氧对这一过程的影响。我们将利用渗透性 膜到EC的原代培养。这个设计是一种 定义心尖室和基底侧室的活体情况。两者都有 酶发光分析与高压液相色谱 技术将被用来测量EC的ATP释放。我们还将 测试ATP释放与释放相关的假设 一氧化氮(NO)。将使用酶发光分析方法来测量 也不是。我们将同时给予ATP和NO的激动剂和拮抗剂 发布并评估各个版本中的任何更改。

DESCRIPTION: Our interest in cardiac EC's stem from the fact that cardiovascular disease continues to be a major health problem in our society. We believe that release of factors from cardiac coronary EC's which participate in the regulation of regional blood flow may have a role in normal cardiac function and in cardiovascular disease. It has been demonstrated that endothelial cells (EC's) release adenosine 5'-triphosphate (ATP) and autocoids outside the cell in response to various stimuli. It has been suggested that ATP release from these cells is receptor-mediated. It is possible that the P2/y receptor plays a role in mediating the ATP release. We want to test the hypothesis that cardiac EC release of ATP in response to agonists occurs both luminally and abluminally and test the effect of hypoxia and re-oxygenation on this process. We will utilize permeable membranes to primary cultures of EC's. This design is a paradigm of the in vivo situation defining apical versus basolateral compartments. Both enzyme luminescence assays and high-pressure liquid chromatography techniques will be used to measure ATP release from EC'S. We will also test the hypothesis that the release of ATP is associated with the release of nitric oxide (NO). Enzyme luminescence assays will be used to measure NO as well. We will administer both agonist and antagonists to ATP and NO release and evaluate any alterations in the respective releases.