AGONIST SPECIFIC ATP RELEASE FROM ENDOTHELIAL CELLS

内皮细胞激动剂特异性 ATP 释放

• 批准号: 6529262
• 负责人: BRIAN C OXHORN
• 金额: $ 1.24万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6529262
• 关键词: adenosine triphosphate; animal tissue; cardiovascular pharmacology; coronary artery; hypoxia; nitric oxide; platelets; receptor; secretion; tissue /cell culture; vascular endothelium; vascular smooth muscle nervous control

DESCRIPTION: Our interest in cardiac EC's stem from the fact that cardiovascular disease continues to be a major health problem in our society. We believe that release of factors from cardiac coronary EC's which participate in the regulation of regional blood flow may have a role in normal cardiac function and in cardiovascular disease. It has been demonstrated that endothelial cells (EC's) release adenosine 5'-triphosphate (ATP) and autocoids outside the cell in response to various stimuli. It has been suggested that ATP release from these cells is receptor-mediated. It is possible that the P2/y receptor plays a role in mediating the ATP release. We want to test the hypothesis that cardiac EC release of ATP in response to agonists occurs both luminally and abluminally and test the effect of hypoxia and re-oxygenation on this process. We will utilize permeable membranes to primary cultures of EC's. This design is a paradigm of the in vivo situation defining apical versus basolateral compartments. Both enzyme luminescence assays and high-pressure liquid chromatography techniques will be used to measure ATP release from EC'S. We will also test the hypothesis that the release of ATP is associated with the release of nitric oxide (NO). Enzyme luminescence assays will be used to measure NO as well. We will administer both agonist and antagonists to ATP and NO release and evaluate any alterations in the respective releases.

产品说明： 我们对心脏EC的兴趣源于心血管 疾病仍然是我们社会的一个主要健康问题。我们认为 从心脏冠状动脉EC释放的因子参与了 局部血流调节可能在正常心脏中起作用 功能和心血管疾病。已经证明 内皮细胞（EC）释放腺苷5 '-三磷酸（ATP）， 细胞外对各种刺激的反应。已经 表明这些细胞释放ATP是受体介导的。是 可能P2/γ受体在介导ATP释放中起作用。 我们想验证心脏EC释放ATP作为反应的假设， 对激动剂的作用在管腔内和管腔外均发生，并测试 缺氧和复氧对这个过程的影响。我们将利用可渗透的 膜到EC的原代培养物。这个设计是一个范例， 定义顶侧与基底外侧室的体内情况。两 酶发光分析和高压液相色谱 技术将用于测量从EC释放的ATP。我们还将 检验ATP的释放与 一氧化氮（NO）酶发光测定将用于测量 也没有。我们将给予ATP和NO的激动剂和拮抗剂 发布并评估相应版本中的任何更改。