INTERMEDIATE FILAMENT--CELL SURFACE INTERACTIONS

中间丝--细胞表面相互作用

• 批准号: 2749838
• 负责人: ROBERT D GOLDMAN
• 金额: $ 26.01万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2749838
• 关键词: active sites; cell cell interaction; cell membrane; cytoskeleton; intermediate filaments; microinjections; molecular cloning; phosphorylation; protein kinase; protein sequence; protein structure function; site directed mutagenesis; tissue /cell culture

DESCRIPTION (Adapted from applicant's abstract): The work described in this project is aimed at determining the structure, properties, and functions of intermediate filaments (IF) in mammalian cells. Many of the experiments take advantage of recently discovered properties of IF showing that they are dynamic cytoskeletal proteins. Dr. Goldman proposes to use specific peptides designed to disrupt IF assemblies in vivo using microinjection methods and to determine the physiological changes which accompany their disassembly. He also proposes to study the mechanisms by which phosphorylation modifies IF organization in cells using a variety of cellular and molecular approaches, including microscopic, biochemical, and immunological techniques. Another major goal is to determine the function of one of the major IF associated proteins, IFAP300. This protein appears to be involved in regulating the distribution and supramolecular organization of IF networks in cells via its crossbridging function. IFAP300 also appears to be involved in interactions with other cytoskeletal systems such as microtubules. In order to further understanding of the function of this protein, it is being cloned and sequenced using standard methods.

描述(改编自申请人的摘要)： 该项目的目的是确定结构、性能和 哺乳动物细胞中中间丝(IF)的功能。许多. 这些实验利用了最近发现的IF的特性 表明它们是动态的细胞骨架蛋白。戈德曼博士 建议使用特定的多肽来破坏IF在 并采用体内微量注射的方法测定生理活性。 伴随着它们的拆卸而来的变化。他还提议研究 磷酸化修饰IF组织的机制 使用各种细胞和分子方法的细胞，包括 显微镜、生化和免疫学技术。另一大专业 目标是确定其中一个主要的功能是否相关联 蛋白质，IFAP300。这种蛋白质似乎参与调节 IF网络在细胞内的分布和超分子组织 它的交叉桥功能。IFAP300似乎也参与了 与微管等其他细胞骨架系统的相互作用。在……里面 为了进一步了解这种蛋白质的功能，它是 使用标准方法进行克隆和测序。