ALTERED SUBSTRATE PREFERENCE IN REPERFUSED HEARTS

描述(改编自申请人摘要)：本申请 研究缺血后心脏活性的标记物，改变 底物偏好。心脏通常使用外源性长链脂肪酸 (LC-FA)作为能源生产的首选底物。LC-FA也可能是 以甘油三酯(TG)的形式存储，但LC-FA的存储速度较低 正常的心脏。因此，正常心脏的甘油三酯含量较低。 交替底物的氧化，如葡萄糖，在正常情况下也很低 红心。存活的缺血后心肌显示一种底物模式 偏好不同于正常心脏。具体来说，葡萄糖的氧化 而依赖糖酵解过程的LC-FA的储存也得到了加强。这些 缺血后存活心肌甘油三酯含量增加 已由组织学、放射性核素、断层扫描和 磁共振成像分析。尽管增强型LC-FA 储存是存活的缺血后心肌的标志之一，但鲜为人知 关于LC-FA贮藏与底物变化的关系 在缺血后心肌中的偏爱。此外，其根本原因(S) 缺血后心肌的底物偏好改变尚不清楚。 因此，该应用程序有两个目标。第一个目标是 彻底定量分离的改变的底物偏好 ~(13)C-磁共振波谱在大鼠心肌缺血后的应用 放射性核素技术。第二个目标是确定潜在的 酶分析中底物偏好改变的原因(S)。 实现这些目标将勾勒出 LC-FA储存增强可作为缺血后心肌存活的标志物 红心。

DESCRIPTION (Adapted from Applicant's Abstract): This application investi-gates a marker of viability in post-ischemic hearts, altered substrate preference. Hearts normally use exogenous long-chain fatty acids (LC-FA) as the preferred substrate for energy production. LC-FA also may be stored as triglycerides (TG) but storage of LC-FA occurs at a low rate in normal hearts. As a result, the TG content of normal hearts is low. Oxidation of alternate substrate, such as glucose also is low in normal hearts. Viable post-ischemic myocardium demonstrates a pattern of substrate preference different from normal hearts. Specifically, oxidation of glucose and storage of LC-FA, a glycolysis-dependent process, are enhanced. These changes increase the content of TG in viable post-ischemic myocardium, an event that has been reported by histological, radionuclide, tomographic and magnetic resonance imaging analyses. Despite the fact that enhanced LC-FA storage is one hallmark of viable post-ischemic myocardium, little is known about the relationship between LC-FA storage and altered substrate preference in post-ischemic myocardium. Also, the underlying cause(s) of altered substrate preference in post-ischemic myocardium is not known. Consequently, the application has two goals. The first goal is to quantitate thoroughly the altered substrate preference in isolated post-ischemic rat hearts using 13C-magnetic resonance spectroscopy and radionuclide technologies. The second goal is to determine the underlying cause(s) for altered substrate preference using enzymatic analyses. Accomplishing these goals will delineate the mechanism and importance of enhanced LC-FA storage as a marker for myocardial viability in post-ischemic hearts.