ALTERED SUBSTRATE PREFERENCE IN REPERFUSED HEARTS

描述（改编自申请人的摘要）：本申请 研究缺血后心脏活力的标志物， 底物偏好 心脏通常使用外源性长链脂肪酸 （LC-FA）作为用于能量产生的优选底物。 LC-FA也可以是 以甘油三酯（TG）形式储存，但LC-FA的储存率较低， 正常的心脏 因此，正常心脏的TG含量较低。 在正常情况下，替代底物（如葡萄糖）的氧化也很低。 心中 存活的缺血后心肌表现出一种基质模式 与正常心脏不同的偏好。 具体来说，葡萄糖的氧化 和LC-FA的储存，糖酵解依赖性过程，得到增强。 这些 改变增加缺血后存活心肌中TG的含量， 组织学、放射性核素、断层扫描和 磁共振成像分析。 尽管增强的LC-FA 储存是缺血后存活心肌的一个标志， 关于LC-FA存储和改变的底物之间的关系 在缺血后心肌中优先。 此外， 在缺血后心肌中改变的底物偏好是未知的。 因此，应用程序有两个目标。 第一个目标是 在分离的细胞中彻底定量改变的底物偏好， 使用13 C-磁共振波谱法的缺血后大鼠心脏， 放射性核素技术。 第二个目标是确定潜在的 使用酶分析改变底物偏好的原因。 实现这些目标将阐明的机制和重要性， 增强的LC-FA储存作为缺血后心肌存活的标志物 心中

DESCRIPTION (Adapted from Applicant's Abstract): This application investi-gates a marker of viability in post-ischemic hearts, altered substrate preference. Hearts normally use exogenous long-chain fatty acids (LC-FA) as the preferred substrate for energy production. LC-FA also may be stored as triglycerides (TG) but storage of LC-FA occurs at a low rate in normal hearts. As a result, the TG content of normal hearts is low. Oxidation of alternate substrate, such as glucose also is low in normal hearts. Viable post-ischemic myocardium demonstrates a pattern of substrate preference different from normal hearts. Specifically, oxidation of glucose and storage of LC-FA, a glycolysis-dependent process, are enhanced. These changes increase the content of TG in viable post-ischemic myocardium, an event that has been reported by histological, radionuclide, tomographic and magnetic resonance imaging analyses. Despite the fact that enhanced LC-FA storage is one hallmark of viable post-ischemic myocardium, little is known about the relationship between LC-FA storage and altered substrate preference in post-ischemic myocardium. Also, the underlying cause(s) of altered substrate preference in post-ischemic myocardium is not known. Consequently, the application has two goals. The first goal is to quantitate thoroughly the altered substrate preference in isolated post-ischemic rat hearts using 13C-magnetic resonance spectroscopy and radionuclide technologies. The second goal is to determine the underlying cause(s) for altered substrate preference using enzymatic analyses. Accomplishing these goals will delineate the mechanism and importance of enhanced LC-FA storage as a marker for myocardial viability in post-ischemic hearts.