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CARNITINE TRANSPORT IN NATURAL AND ARTIFICIAL MEMBRANE

CARNITINE TRANSPORT IN NATURAL AND ARTIFICIAL MEMBRANE
天然和人造膜中肉碱的转运
批准号:
3354744
负责人:
PAUL E WOLKOWICZ
金额:
$9.15万
依托单位国家:
美国
项目类别:
财政年份:
1986
资助国家:
美国
项目状态:
已结题
起止时间:
1986-08-01 至 1990-07-31

项目摘要

项目成果

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中文摘要
翻译
这一建议的长期目标是阐明机制
英文摘要
The long term objectives of this proposal are to elucidate the mechanism of acylcarnitine translocation across the mitochondrial inner membrane and to determine factors which interact with the translocase and control acylcarnitine cellular distribution. The overall specific aims are to determine structural and functional properties of the translocase isolated from beef heart submitochondrial particles. The translocase will be purified on a carnitine affinity column and activity reconstituted into phospholipid vesicles. Physical characterization of the isolated protein(s) will include: molecular weight, subunit composition, inhibitor-binding peptides, stoichiometry and affinity of substrate sites; as well as the transbilayer orientation in the presence or absence of substrate using the hydrophilic surface probe, 3-Azido-2,7-Naphthalene Disulfonate. Exchange and/or unidirectional transport activities will be determined. Reconstitution of the translocase will be otpimezed for phospholipid class and acyl chain saturation. The effect of the translocase on intervesicular transfer of acylcarnitine and interaction with fatty acid binding protein will be determined using the concentration sensitive probe, pyrenedodecanoyl-carnitine. Finally, the functional relationship of the translocase with purified carnitine palmitoyltransferase will be determined in phospholipid vesicles by fluorescent energy transfer techniques and by determination of [14C] carnitine release from translocated palmitoyl[14C]carnitine. Studies on the purified translocase are relevant to disease-associated impairment in either the translocase or translocase/transferase coupling, e.g., lipid storage myopathies, muscle dystrophy and certain cardiomyopathies.
期刊论文(5)
专著(0)
科研奖励(0)
会议论文
Carnitine-acylcarnitine translocase in ischemia: evidence for sulfhydryl modification.
缺血中的肉碱-酰基肉碱转位酶:巯基修饰的证据。
DOI: 10.1152/ajpheart.1987.253.6.h1557
发表时间: 1987
期刊: The American journal of physiology
影响因子: --
作者: [Pauly,DF, Yoon,SB, McMillin,JB]
通讯作者: McMillin,JB
The role of calcium in the control of respiration by muscle mitochondria.
钙在肌肉线粒体控制呼吸中的作用。
DOI: --
发表时间: 1989
期刊: Medicine and science in sports and exercise
影响因子: 4.1
作者: [McMillin,JB, Madden,MC]
通讯作者: Madden,MC
Chymotrypsin activates cardiac mitochondrial carnitine-acylcarnitine translocase.
胰凝乳蛋白酶激活心脏线粒体肉碱-酰基肉碱转位酶。
DOI: 10.1042/bj2610363
发表时间: 1989
期刊: The Biochemical journal
影响因子: --
作者: [Wolkowicz,PE, Pauly,DF, VanWinkle,WB, McMillin,JB]
通讯作者: McMillin,JB
Importance of acyl-CoA availability in interpretation of carnitine palmitoyltransferase I kinetics.
酰基辅酶 A 可用性在解释肉碱棕榈酰转移酶 I 动力学中的重要性。
DOI: --
发表时间: 1988
期刊: The Journal of biological chemistry
影响因子: --
作者: [Pauly,DF, McMillin,JB]
通讯作者: McMillin,JB
ALTERED SUBSTRATE PREFERENCE IN REPERFUSED HEARTS
ALTERED SUBSTRATE PREFERENCE IN REPERFUSED HEARTS
ALTERED SUBSTRATE PREFERENCE IN REPERFUSED HEARTS
ALTERED SUBSTRATE PREFERENCE IN REPERFUSED HEARTS
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