NMR INVESTIGATION OF HUMAN CD58 CELL SURFACE RECEPTOR

人 CD58 细胞表面受体的 NMR 研究

• 批准号: 2857024
• 负责人: ZHEN-YU J SUN
• 金额: $ 3.67万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2857024
• 关键词: CD2 molecule; binding sites; human tissue; intermolecular interaction; leukocyte adhesion molecules; nuclear magnetic resonance spectroscopy; protein structure function; site directed mutagenesis

The purpose of this research is to learn about the basic principles involved in the interactions among cell surface receptors. The proposed research will study the mechanism and specificity of the interaction between the human leukocyte CD2 and CD58 cell surface adhesion receptors, using modern NMR spectroscopy and genetic engineering. Human leukocyte cell surface receptors are responsible for many biological processes such as cell adhesion, cell signaling and cell recognition, which are important in development, immune responses, tumor metastasis and wound healing. Our initial research will focus on the NMR structure determination of the adhesion domain of human CD58 receptor. Mutational studies of human CD2 and CD58 receptors will be carried out to study the binding site and binding specificity of the CD2/CD58 interactions, along with the NMR structure determination of the complex between the adhesion domains of CD2 and CD58. This research study will increase our understandings in immunology and cell biology, and lead potentially to the discovery of drugs that can be used in disease control by affecting specific binding activities of cell surface receptors.

本研究的目的是了解基本原理 参与细胞表面受体之间的相互作用。拟议 研究将研究相互作用的机制和特异性 在人白细胞CD 2和CD 58细胞表面粘附受体之间， 使用现代核磁共振光谱和基因工程。人白细胞 表面受体负责许多生物过程， 细胞粘附、细胞信号传导和细胞识别，这些在 发育、免疫应答、肿瘤转移和伤口愈合。我们 初步研究将集中在NMR结构测定的 人CD 58受体的粘附结构域。人CD 2和CD 3的突变研究 将进行CD 58受体的结合位点和结合 CD 2/CD 58相互作用的特异性，沿着NMR结构 确定CD 2和CD 58的粘附结构域之间的复合物。 这项研究将增加我们对免疫学和细胞学的理解， 生物学，并可能导致发现药物，可用于 通过影响细胞表面特异性结合活性的疾病控制 受体。