BREATH-AMMONIA METHOD FOR H. PYLORI DETECTION

该项目将开发一种基于测量患者呼气中的氨气来非侵入性诊断胃内活动性幽门螺杆菌感染的简单测试方法。一种新的高灵敏度光学技术将被用于测量呼气氨，导致一种廉价的诊断程序，可以在任何全科医生的办公室进行。一种改进的检测胃幽门螺杆菌的方法具有临床意义，因为这种细菌最明显的原因是多肽溃疡以及包括癌症在内的其他胃肠道疾病。新的呼气氨传感方法将有助于感染患者的诊断，以及治疗后感染状态的后续确定。这项研究将通过使用当前研究仪器的有限临床试验来证明第一阶段基于氨的技术的可行性。这项工作将包括研究，确定和改进传感方法，并使临床方案合格。随后的第二阶段研究将完善传感系统，建立临床水平的原型仪器。将进行扩大的患者试验，以确认该方案和改进的诊断系统的临床效果。拟议的商业应用：传感技术主要设计用于胃幽门螺杆菌的临床诊断和相关疾病的应用。它还适用于在与氨升高相关的疾病状态(例如终末期肾功能衰竭)的情况下进行氨检测。作为一种高灵敏度的氨气检测系统，该技术在工业和食品行业的氨气传感应用中具有很高的应用价值。

This project will develop a simple test for noninvasive diagnosis of active Helicobacter pylori infection in the stomach based on measuring ammonia in patients' breath. A novel highly sensitive optical technique will be used to measure breath ammonia, leading to an inexpensive diagnostic procedure that can be conducted in any general practitioner's office. An improved method of detecting gastric H. pylori is of clinical significance because the organism causes most notably peptide ulcers as well as other gastrointestinal diseases including cancers. The new breath ammonia sensing method will be useful for diagnosis of infected patients, as well as follow-up determinations of infectious status after therapy. The research will demonstrate the feasibility of the ammonia-based technique in Phase I through a limited clinical trial using current research instrumentation. This effort will include investigations characterizing and improving the sensing method and qualifying the clinical protocol. Subsequent Phase II research will refine the sensing system building clinical level prototype instruments. Expanded patient trials will be conducted to confirm the clinical efficacy of the protocol and improved diagnostic system. PROPOSED COMMERCIAL APPLICATIONS: The sensing technique is primarily designed for clinical diagnosis of gastric H. pylori and related disease applications. It is also applicable towards ammonia detection in context of their disease states associated with elevated ammonia, e.g. end-stage renal failure. As a high-sensitivity ammonia detection system, the technology is valuable for sensing ammonia in industrial and food industry applications.