OXYGEN BIO-ANALYZER USING OPTICAL SENSOR TECHNIQUES

该项目的目标是开发和生产一种光学 基于纤维的氧微传感器，其在体外对 细胞培养物中的氧浓度。 这个传感器系统的价值 是在生物测定的基础上测量氧代谢的细胞培养，细胞 毒性研究、药物测试和环境质量测试。 第一阶段的目的是测试传感器设计的可行性， 准确测定细胞氧呼吸的速率和质量 在正常生长期间和在各种毒性或刺激条件下。 该传感器通过将磷光指示剂化合物附着到 光纤的末端 激发光投射通过 光纤激发磷光体， 衰变信号返回到探测器。 磷光体的发射受到影响 通过存在猝灭长寿命发光的氧。 因此，在光学上跟踪氧气中的氧气水平是实际的。 在传感器尖端的细胞的微环境的基础上的寿命 磷光 开发的技术将产生一系列通用的生物测定产品 其应用范围包括细胞代谢、毒理学、癌症 从生物学、治疗学的发展、诊断学到临床医学。

The project's objective is the development and production of an optical fiber-based oxygen microsensor which is sensitive in vitro to changes in oxygen concentration within cell cultures. The value of this sensor system is in bioassays based on measuring oxygen metabolism in cell cultures, cell toxicity studies, drug testing and environment quality testing. The aims of Phase I are to test the feasibility of the sensor design for accurately determining the rate and quality of cells' oxygen respiration during normal growth and under various toxic or stimulatory conditions. The sensor is fabricated by affixing a phosphorescent indicator compound to the distal tip of a fiber optic. Excitation light projected through the fiber excites the phosphor which emits a wavelength shifted long-lifetime decay signal returned to a detector. The phosphor's emission is affected by the presence of oxygen which quenches the long-lifetime luminescence. It is thereby practical to optically track oxygen levels in the microenvironment of cells at the sensor tip based on the lifetime of the phosphorescence. The technology developed will yield a family of versatile bioassay products with applications that span research in cell metabolism, toxicology, cancer biology, development of therapeutics, and diagnostics to clinical medicine.